BMRBj - BMREntryMaster

Found 1 Document (1.356 seconds)

BMRB: 1451

2DGC

Solution Structure of the Basic Region from the Transcriptional Activator GCN4

Authors

Saudek, V., Pasley, H.S., Gibson, T., Gausepohl, H., Frank, R., Pastore, A.

Assembly

transcriptional regulator GCN4

Entity

1. transcriptional regulator GCN4 (polymer), 61 monomers, 7114.073 Da Detail

VPESSDPAAL KRARNTEAAR RSRARKLQRM KQLEDKVEEL LSKNYHLENE VARLKKLVGE R

Formula weight

7114.073 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 60.7 %, Completeness: 59.1 %, Completeness (bb): 57.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	59.1 % (234 of 396)	59.1 % (234 of 396)
Backbone	57.9 % (70 of 121)	57.9 % (70 of 121)
Sidechain	59.6 % (164 of 275)	59.6 % (164 of 275)
Aromatic	0.0 % (0 of 6)	0.0 % (0 of 6)
Methyl	43.8 % (14 of 32)	43.8 % (14 of 32)

1. transcriptional regulator GCN4

VPESSDPAAL KRARNTEAAR RSRARKLQRM KQLEDKVEEL LSKNYHLENE VARLKKLVGE R

Show sample condition

Sample

Temperature 310 K, pH 3.1

Release date

1995-07-30

Citation

Solution structure of the basic region from the transcriptional activator GCN4
Saudek, V., Pasley, H.S., Gibson, T., Gausepohl, H., Frank, R., Pastore, A.
Biochemistry (1991), 30, 1310-1317, PubMed 1991112 , DOI: , Abstract

Related entities 1. transcriptional regulator GCN4, : 1 : 3 : 2 : 89 entities Detail

Interaction partners 1. transcriptional regulator GCN4, : 13 interactors Detail

More details for 13 interactors identified by 9 citations

Experiments performed 1 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr1451_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr1451_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chemical_shift_assignment_data_set_one	Warning	266		59.0 (chain: 1, length: 61)

Assigned chemical shifts

Saveframe: chemical_shift_assignment_data_set_one
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 61, Sequence coverage: 59.0%
- Total number of assignments
  - ¹H chemical shifts: 266
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
11	LYS	HZ1	7.5
11	LYS	HZ2	7.5
11	LYS	HZ3	7.5
26	LYS	HZ1	7.4
26	LYS	HZ2	7.4
26	LYS	HZ3	7.4

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Found 1 Document (1.356 seconds)

BMRB: 1451

Sample

Related entities 1. transcriptional regulator GCN4, : 1 : 3 : 2 : 89 entities Detail

Interaction partners 1. transcriptional regulator GCN4, : 13 interactors Detail

Experiments performed 1 experiments Detail

Instrument

Sample

Chemical shift validation 3 contents Detail

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
11	LYS	HZ1	7.5
11	LYS	HZ2	7.5
11	LYS	HZ3	7.5
26	LYS	HZ1	7.4
26	LYS	HZ2	7.4
26	LYS	HZ3	7.4

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
11	LYS	HZ1	7.5
11	LYS	HZ2	7.5
11	LYS	HZ3	7.5
26	LYS	HZ1	7.4
26	LYS	HZ2	7.4
26	LYS	HZ3	7.4

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
11	LYS	HZ1	7.5
11	LYS	HZ2	7.5
11	LYS	HZ3	7.5
26	LYS	HZ1	7.4
26	LYS	HZ2	7.4
26	LYS	HZ3	7.4