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Assignment of the 1H-NMR spectrum of a lac repressor headpiece-operator complex in H2O and identification of NOEs
Authors
Lamerichs, R.M.J.N., Boelens, R., van der Marel, G.A., van Boom, J.H., Kaptein, R.
Assembly
lac repressor headpiece
Entity
1. lac repressor headpiece (polymer), 56 monomers, 6226.011 Da Detail

MKPVTLYDVA EYAGVSYQTV SRVVNQASHV SAKTREKVEA AMAELNYIPN RVAQQL


Formula weight
6226.011 Da
Source organism
Escherichia coli
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 94.6 %, Completeness: 68.1 %, Completeness (bb): 89.1 % Detail

Polymer type: polypeptide(L)

Total1H
All68.1 % (224 of 329)68.1 % (224 of 329)
Backbone89.1 % (98 of 110)89.1 % (98 of 110)
Sidechain57.5 % (126 of 219)57.5 % (126 of 219)
Aromatic100.0 % (18 of 18)100.0 % (18 of 18)
Methyl94.6 % (35 of 37)94.6 % (35 of 37)

1. lac repressor headpiece

MKPVTLYDVA EYAGVSYQTV SRVVNQASHV SAKTREKVEA AMAELNYIPN RVAQQL

Sample

Temperature 300 K, pH 6



Release date
1995-07-30
Citation
Assignment of the 1H-NMR spectrum of a lac repressor headpiece-operator complex in H2O and identification of NOEs. Consequences for protein-DNA interaction
Lamerichs, R.M.J.N., Boelens, R., van der Marel, G.A., van Boom, J.H., Kaptein, R.
Eur. J. Biochem. (1990), 194, 629-637, PubMed 2269288 , DOI 10.1111/j.1432-1033.1990.tb15662.x ,
Related entities 1. lac repressor headpiece, : 1 : 10 : 2 : 9 : 144 entities Detail
Interaction partners 1. lac repressor headpiece, : 6 interactors Detail
Experiments performed 1 experiments Detail