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Structures and chemical shift assignments for the ADD domain of the ATRX protein
Authors
Yang, J., Neuhaus, D.
Assembly
ADD domain
Entity
1. ADD domain 156-296 (polymer, Thiol state: free and other bound), 142 monomers, 16254.43 Da Detail

GAMADKRGDG LHGIVSCTAC GQQVNHFQKD SIYRHPSLQV LICKNCFKYY MSDDISRDSD GMDEQCRWCA EGGNLICCDF CHNAFCKKCI LRNLGRKELS TIMDENNQWY CYICHPEPLL DLVTACNSVF ENLEQLLQQN KK


2. ZN (non-polymer), 65.409 × 3 Da
Total weight
16450.656 Da
Max. entity weight
16254.43 Da
Entity Connection
na 12 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1nasing1:CYS17:SG2:ZN1:ZN
2nasing1:CYS20:SG2:ZN1:ZN
3nasing1:CYS43:SG2:ZN1:ZN
4nasing1:CYS46:SG2:ZN1:ZN
5nasing1:CYS66:SG2:ZN1:ZN
6nasing1:CYS69:SG2:ZN1:ZN
7nasing1:CYS86:SG2:ZN1:ZN
8nasing1:CYS89:SG2:ZN1:ZN
9nasing1:CYS78:SG2:ZN1:ZN
10nasing1:CYS81:SG2:ZN1:ZN
11nasing1:CYS111:SG2:ZN1:ZN
12nasing1:CYS114:SG2:ZN1:ZN

Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 99.3 %, Completeness: 77.3 %, Completeness (bb): 82.2 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All77.3 % (1280 of 1656)84.0 % (725 of 863)66.2 % (415 of 627)84.3 % (140 of 166)
Backbone82.2 % (688 of 837)95.7 % (269 of 281)68.1 % (284 of 417)97.1 % (135 of 139)
Sidechain75.6 % (720 of 952)78.4 % (456 of 582)75.5 % (259 of 343)18.5 % (5 of 27)
Aromatic84.3 % (113 of 134)94.0 % (63 of 67)73.8 % (48 of 65)100.0 % (2 of 2)
Methyl98.4 % (120 of 122)96.7 % (59 of 61)100.0 % (61 of 61)

1. ADD domain 156-296

GAMADKRGDG LHGIVSCTAC GQQVNHFQKD SIYRHPSLQV LICKNCFKYY MSDDISRDSD GMDEQCRWCA EGGNLICCDF CHNAFCKKCI LRNLGRKELS TIMDENNQWY CYICHPEPLL DLVTACNSVF ENLEQLLQQN KK

Sample

Pressure 1 atm, Temperature 300 K, pH 6.7


#NameIsotope labelingTypeConcentration
1ADD domain 156-296[U-15N]0.6 ~ 0.8 mM
2TRISnone20 mM
3DTTnone1 mM
4zinc chloridenone100 uM
5sodium chloridenone0.5 M

LACS Plot; CA
Referencing offset: -0.03 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: -0.03 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: -0.06 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 34 models in PDB: 2LD1, Strand ID: A Detail


Release date
2007-06-17
Citation
Structural consequences of disease-causing mutations in the ATRX-DNMT3-DNMT3L (ADD) domain of the chromatin-associated protein ATRX
Argentaro, A., Yang, J., Chapman, L., Kowalczyk, M.S., Gibbons, R.J., Higgs, D.R., Neuhaus, D., Rhodes, D.
Proc. Natl. Acad. Sci. U. S. A. (2007), 104, 11939-11944, PubMed 17609377 , DOI 10.1073/pnas.0704057104 ,
Entries sharing articles Swiss-Prot: 1 entries Detail
  Swiss-Prot: P46100 released on 1995-11-01
    Title ATRX_HUMAN Entity Transcriptional regulator ATRX
Related entities 1. ADD domain 156-296, : 1 : 1 : 14 entities Detail
Interaction partners 1. ADD domain 156-296, : 33 interactors Detail
Experiments performed 15 experiments Detail
Keywords ADD domain