BMRBj - BMREntryMaster

Found 1 Document (0.474 seconds)

BMRB: 15019

3ZE4

NMR assignments for a helical diacylglycerol kinase (DAGK), 40 kDa membrane protein from E. coli

Authors

Kim, H., Oxenoid, K., Jacob, J., Sonnichsen, F.D., Sanders, C.R.

Assembly

DAGK homotrimer

Entity

1. DAGK homotrimer (polymer, Thiol state: all free), 122 monomers, 13244.52 × 3 Da Detail

MANNTTGFTR IIKAAGYSWK GLRAAWINEA AFRQEGVAVL LAVVIACWLD VDAITRVLLI SSVMLVMIVE ILNSAIEAVV DRIGSEYHEL SGRAKDMGSA AVLIAIIVAV ITWCILLWSH FG

Total weight

39733.56 Da

Max. entity weight

13244.52 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.7 %, Completeness: 42.5 %, Completeness (bb): 74.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	42.5 % (591 of 1390)	21.2 % (148 of 698)	59.6 % (334 of 560)	82.6 % (109 of 132)
Backbone	74.7 % (547 of 732)	46.4 % (117 of 252)	89.7 % (321 of 358)	89.3 % (109 of 122)
Sidechain	18.9 % (146 of 772)	7.0 % (31 of 446)	36.4 % (115 of 316)	0.0 % (0 of 10)
Aromatic	0.0 % (0 of 114)	0.0 % (0 of 57)	0.0 % (0 of 52)	0.0 % (0 of 5)
Methyl	21.0 % (44 of 210)	14.3 % (15 of 105)	27.6 % (29 of 105)

1. DAGK

MANNTTGFTR IIKAAGYSWK GLRAAWINEA AFRQEGVAVL LAVVIACWLD VDAITRVLLI SSVMLVMIVE ILNSAIEAVV DRIGSEYHEL SGRAKDMGSA AVLIAIIVAV ITWCILLWSH FG

Show sample condition

Sample

Temperature 318 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	DAGK	[U-13C; U-15N; U-2H]		3 mM

Release date

2006-12-05

Citation

NMR assignments for a helical 40 kDa membrane protein
Oxenoid, K., Kim, H., Jacob, J., Sonnichsen, F., Sanders, C.
J. Am. Chem. Soc. (2004), 126, 5048-5049, PubMed 15099070 , DOI 10.1021/ja049916m , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 7340 released on 2006-12-05
Title NMR assignments for a helical diacylglycerol kinase (DAGK), 40 kDa membrane protein from E. coli

Related entities 1. DAGK homotrimer, : 1 : 2 : 1 : 1 : 25 entities Detail

Experiments performed 5 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr15019_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr15019_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	OK	538		96.7 (chain: 1, length: 122)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 122, Sequence coverage: 96.7%
- Total number of assignments
  - ¹H chemical shifts: 109
  - ¹³C chemical shifts: 320
  - ¹⁵N chemical shifts: 109
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

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Found 1 Document (0.474 seconds)

BMRB: 15019

Sample

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. DAGK homotrimer, : 1 : 2 : 1 : 1 : 25 entities Detail

Experiments performed 5 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	698	109	15.6
¹³C chemical shifts	560	320	57.1
¹⁵N chemical shifts	138	109	79.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	252	109	43.3
¹³C chemical shifts	244	218	89.3
¹⁵N chemical shifts	122	109	89.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	446	0	0.0
¹³C chemical shifts	316	102	32.3
¹⁵N chemical shifts	16	0	0.0