Search

Ubiquitin in 8 M Urea
Authors
Meier, S., Strohmeier, M., Blackledge, M., Grzesiek, S.
Assembly
denatured ubiquitin
Entity
1. denatured ubiquitin (polymer, Thiol state: not present), 76 monomers, 8564.731 Da Detail

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG


Formula weight
8564.731 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 98.7 %, Completeness: 25.1 %, Completeness (bb): 48.4 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All25.1 % (229 of 913)16.9 % (81 of 478)21.4 % (75 of 350)85.9 % (73 of 85)
Backbone48.4 % (215 of 444)46.3 % (69 of 149)33.3 % (74 of 222)98.6 % (72 of 73)
Sidechain 2.8 % (15 of 539) 3.6 % (12 of 329) 1.0 % (2 of 198) 8.3 % (1 of 12)
Aromatic 0.0 % (0 of 32) 0.0 % (0 of 16) 0.0 % (0 of 16)
Methyl 0.0 % (0 of 98) 0.0 % (0 of 49) 0.0 % (0 of 49)

1. denatured ubiquitin

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

Sample

Pressure 1 atm, Temperature 298 K, pH 2.5


#NameIsotope labelingTypeConcentration
1denatured ubiquitin[U-13C; U-15N; U-2H]2.5 mM
2H2Onatural abundance55.5 M
3D2O[U-2H]5 %
4ureanatural abundance8 M

Release date
2006-12-10
Citation
Direct observation of dipolar couplings and hydrogen bonds across a beta-hairpin in 8 M urea
Meier, S., Strohmeier, M., Blackledge, M., Grzesiek, S.
J. Am. Chem. Soc. (2007), 129, 754-755, PubMed 17243799 , DOI 10.1021/ja067522k
Related entities 1. denatured ubiquitin, : 1 : 200 : 5 : 25 : 139 entities Detail
Interaction partners 1. denatured ubiquitin, : 24 interactors Detail
Experiments performed 7 experiments Detail