BMRBj - BMREntryMaster

Found 1 Document (2.269 seconds)

BMRB: 15047

8J1P

Ubiquitin in 8 M Urea

Authors

Meier, S., Strohmeier, M., Blackledge, M., Grzesiek, S.

Assembly

denatured ubiquitin

Entity

1. denatured ubiquitin (polymer, Thiol state: not present), 76 monomers, 8564.731 Da Detail

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

Formula weight

8564.731 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.7 %, Completeness: 25.4 %, Completeness (bb): 48.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	25.4 % (231 of 909)	18.2 % (87 of 478)	20.6 % (72 of 350)	88.9 % (72 of 81)
Backbone	48.7 % (219 of 450)	48.4 % (75 of 155)	32.4 % (72 of 222)	98.6 % (72 of 73)
Sidechain	2.5 % (13 of 529)	3.7 % (12 of 323)	0.5 % (1 of 198)	0.0 % (0 of 8)
Aromatic	0.0 % (0 of 32)	0.0 % (0 of 16)	0.0 % (0 of 16)
Methyl	4.1 % (4 of 98)	8.2 % (4 of 49)	0.0 % (0 of 49)

1. denatured ubiquitin

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 2.5

#	Name	Isotope labeling	Concentration
1	denatured ubiquitin	[U-13C; U-15N; U-2H]	2.5 mM
2	H2O	natural abundance	55.5 M
3	D2O	[U-2H]	5 %
4	urea	natural abundance	8 M

Release date

2006-12-10

Citation

Direct observation of dipolar couplings and hydrogen bonds across a beta-hairpin in 8 M urea
Meier, S., Strohmeier, M., Blackledge, M., Grzesiek, S.
J. Am. Chem. Soc. (2007), 129, 754-755, PubMed 17243799 , DOI 10.1021/ja067522k , Abstract

Related entities 1. denatured ubiquitin, : 1 : 292 : 11 : 71 : 321 entities Detail

Interaction partners 1. denatured ubiquitin, : 27 interactors Detail

More details for 27 interactors identified by 16 citations

Experiments performed 7 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr15047_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr15047_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	OK	215		98.7 (chain: 1, length: 76)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 76, Sequence coverage: 98.7%
- Total number of assignments
  - ¹³C chemical shifts: 71
  - ¹H chemical shifts: 72
  - ¹⁵N chemical shifts: 72
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	350	71	20.3
¹H chemical shifts	478	72	15.1
¹⁵N chemical shifts	85	72	84.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	152	71	46.7
¹H chemical shifts	155	72	46.5
¹⁵N chemical shifts	73	72	98.6

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Found 1 Document (2.269 seconds)

BMRB: 15047

Sample

Related entities 1. denatured ubiquitin, : 1 : 292 : 11 : 71 : 321 entities Detail

Interaction partners 1. denatured ubiquitin, : 27 interactors Detail

Experiments performed 7 experiments Detail

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Chemical shift validation 3 contents Detail