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BMRB: 15049

2JZX

Resonance assignment of the first and second KH (hnRNP-K homology) domains of human poly(C)-binding protein-2 (PCBP2)

Authors

Du, Z., Fenn, S., James, T.L.

Assembly

PCBP2 KH1+KH2

Entity

1. PCBP2 KH1+KH2 (polymer, Thiol state: all free), 168 monomers, 18060.96 Da Detail

MKHHHHHHKN VTLTIRLLMH GKEVGSIIGK KGESVKKMRE ESGARINISE GNCPERIITL AGPTNAIFKA FAMIIGKLEE DISSSMTNST AASRPPVTLR LVVPASQCGS LIGKGGCKIK EIRESTGAQV QVAGDMLPNS TERAITIAGI PQSIIECVKQ ICVVMLET

Formula weight

18060.96 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.8 %, Completeness: 87.7 %, Completeness (bb): 92.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.7 % (1663 of 1897)	87.2 % (862 of 988)	86.2 % (635 of 737)	96.5 % (166 of 172)
Backbone	92.5 % (919 of 994)	92.2 % (317 of 344)	91.4 % (447 of 489)	96.3 % (155 of 161)
Sidechain	83.2 % (879 of 1056)	84.6 % (545 of 644)	80.5 % (323 of 401)	100.0 % (11 of 11)
Aromatic	4.2 % (2 of 48)	8.3 % (2 of 24)	0.0 % (0 of 24)
Methyl	98.1 % (206 of 210)	98.1 % (103 of 105)	98.1 % (103 of 105)

1. PCBP2 KH1 and KH2 domains

MKHHHHHHKN VTLTIRLLMH GKEVGSIIGK KGESVKKMRE ESGARINISE GNCPERIITL AGPTNAIFKA FAMIIGKLEE DISSSMTNST AASRPPVTLR LVVPASQCGS LIGKGGCKIK EIRESTGAQV QVAGDMLPNS TERAITIAGI PQSIIECVKQ ICVVMLET

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.4

#	Name	Isotope labeling	Concentration
1	PCBP2 KH1 and KH2 domains	[U-100% 13C; U-100% 15N]	1 mM
2	H2O		10 %
3	D2O		90 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.17 ppm, Outliers: 3 Detail

Release date

2007-05-08

Citation

Resonance assignment of the first and second KH (hnRNP-K homology) domains of human poly(C)-binding protein-2 (PCBP2)
Du, Z., Fenn, S., James, T.L.
J. Biomol. NMR (2007), 38, 197-197, PubMed 17479348 , DOI 10.1007/s10858-007-9160-z , Abstract

Related entities 1. PCBP2 KH1+KH2, : 1 : 3 : 212 entities Detail

Interaction partners 1. PCBP2 KH1+KH2, : 74 interactors Detail

More details for 74 interactors identified by 35 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC