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BMRB: 15070

3I8R

NMR Assignments of the apo Corynebacterium diphtheria Heme Oxygenase-G135A

Authors

Hom, K.

Assembly

heme oxygenase

Entity

1. heme oxygenase (polymer, Thiol state: not present), 215 monomers, 24151.89 Da Detail

MTTATAGLAV ELKQSTAQAH EKAEHSTFMS DLLKGRLGVA EFTRLQEQAW LFYTALEQAV DAVRASGFAE SLLDPALNRA EVLARDLDKL NGSSEWRSRI TASPAVIDYV NRLEEIRDNV DGPALVAHHY VRYLADLSGG QVIARMMQRH YGVDPEALGF YHFEGIAKLK VYKDEYREKL NNLELSDEQR EHLLKEATDA FVFNHQVFAD LGKGL

Formula weight

24151.89 Da

Source organism

Corynebacterium diphtheriae

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.3 %, Completeness: 44.3 %, Completeness (bb): 77.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	44.3 % (1099 of 2479)	23.5 % (300 of 1278)	62.6 % (608 of 972)	83.4 % (191 of 229)
Backbone	77.0 % (987 of 1282)	50.3 % (221 of 439)	91.0 % (575 of 632)	90.5 % (191 of 211)
Sidechain	20.7 % (290 of 1399)	9.4 % (79 of 839)	38.9 % (211 of 542)	0.0 % (0 of 18)
Aromatic	0.0 % (0 of 210)	0.0 % (0 of 105)	0.0 % (0 of 103)	0.0 % (0 of 2)
Methyl	27.3 % (71 of 260)	17.7 % (23 of 130)	36.9 % (48 of 130)

1. heme oxygenase

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	heme oxygenase	[U-13C; U-15N; U-2H]	1.0 ~ 2.0 mM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %
4	potassium phosphate	natural abundance	50 mM
5	potassium chloride	natural abundance	100 mM
6	DSS	natural abundance	0.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.72 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.72 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.62 ppm, Outliers: 3 Detail

Release date

2007-06-25

Citation

NMR assignments of cd-HO, a 24 kDa heme oxygenase from Corynebacterium diphtheria
Hom, K., Furci, L.M., Deshmukh, R., Wilks, A.
Biomol. NMR Assign. (2007), 1, 55-56, PubMed 19636825 , DOI 10.1007/s12104-007-9014-3 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 15071 released on 2007-06-25
    Title NMR Assignments of the apo Corynebacterium diphtheria Heme Oxygenase
  BMRB: 15073 released on 2007-06-25
    Title NMR assignments of CO-bound ferrous Corynebacterium diphtheria Heme Oxygenase

Related entities 1. heme oxygenase, : 1 : 13 : 101 entities Detail

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC