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The solution structure of the monomeric species of the C terminal domain of the CA protein of HIV-1
Authors
Alcaraz, L.A., del Alamo, M., Barrera, F.N., Mateu, M.G., Neira, J.L.
Assembly
CAC monomer
Entity
1. CAC monomer (polymer, Thiol state: all free), 87 monomers, 9548.908 Da Detail

MSPTSILDIR QGPKEPFRDY VDRFYKTLRA EQASQEVKNA MTETLLVQNA NPDCKTILKA LGPAATLEEM MTACQGVGGP GHKARVL


Formula weight
9548.908 Da
Source organism
Human immunodeficiency virus 1
Exptl. method
SOLUTION NMR
Refine. method
molecular dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 94.3 %, Completeness: 81.3 %, Completeness (bb): 83.9 % Detail

Polymer type: polypeptide(L)

Total1H15N
All81.3 % (499 of 614)82.7 % (430 of 520)73.4 % (69 of 94)
Backbone83.9 % (209 of 249)83.3 % (140 of 168)85.2 % (69 of 81)
Sidechain79.5 % (290 of 365)82.4 % (290 of 352) 0.0 % (0 of 13)
Aromatic95.0 % (19 of 20)95.0 % (19 of 20)
Methyl97.9 % (47 of 48)97.9 % (47 of 48)

1. CAC monomer

MSPTSILDIR QGPKEPFRDY VDRFYKTLRA EQASQEVKNA MTETLLVQNA NPDCKTILKA LGPAATLEEM MTACQGVGGP GHKARVL

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7


#NameIsotope labelingTypeConcentration
1entity[U-15N]1 mM
2potassium phosphatenone100 mM
3TSPnone0.00001 mM

Protein Blocks Logo
Calculated from 30 models in PDB: 2JO0, Strand ID: A Detail


Release date
2007-10-23
Citation
Flexibility in HIV-1 assembly subunits: solution structure of the monomeric C-terminal domain of the capsid protein
Alcaraz, L.A., del Alamo, M., Barrera, F.N., Mateu, M.G., Neira, J.L.
Biophys. J. (2007), 93, 1264-1276, PubMed 17526561 , DOI 10.1529/biophysj.106.101089 ,
Related entities 1. CAC monomer, : 1 : 113 : 87 entities Detail
Interaction partners 1. CAC monomer, : 1 interactors Detail
Experiments performed 5 experiments Detail
Keywords CAPSIS PROTEIN, HIV, MONOMER