13C and 15N resonance assignments for the major coat protein in Pf1 filamentous bacteriophage
Polymer type: polypeptide(L)
Total | 13C | 15N | |
---|---|---|---|
All | 93.4 % (226 of 242) | 92.3 % (180 of 195) | 97.9 % (46 of 47) |
Backbone | 98.3 % (174 of 177) | 98.5 % (129 of 131) | 97.8 % (45 of 46) |
Sidechain | 86.5 % (90 of 104) | 86.4 % (89 of 103) | 100.0 % (1 of 1) |
Aromatic | 25.0 % (2 of 8) | 25.0 % (2 of 8) | |
Methyl | 87.2 % (34 of 39) | 87.2 % (34 of 39) |
1. Pf1 phage coat protein
GVIDTSAVES AITDGQGDMK AIGGYIVGAL VILAVAGLIY SMLRKATemperature 297.5 (±12.5) K, pH 8.4, Details Precipitated, hydrated. The sample weight is 7-10 mg.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Pf1 phage | [U-13C; U-15N] | 7.0 ~ 10.0 mg |
Temperature 297.5 (±12.5) K, pH 8.4, Details Precipitated, hydrated. The sample weight is 7-10 mg. The sample is prepared with glucose labeled only on carbon-1 position, and it results in labeling of most but not all carbonyls.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
2 | Pf1 phage | [U-15N; 13C C1-Glucose] | 7.0 ~ 10.0 mg |
Polymer type: polypeptide(L)
Total | 13C | 15N | |
---|---|---|---|
All | 50.8 % (246 of 484) | 50.3 % (196 of 390) | 53.2 % (50 of 94) |
Backbone | 53.7 % (190 of 354) | 53.8 % (141 of 262) | 53.3 % (49 of 92) |
Sidechain | 47.1 % (98 of 208) | 47.1 % (97 of 206) | 50.0 % (1 of 2) |
Aromatic | 12.5 % (2 of 16) | 12.5 % (2 of 16) | |
Methyl | 50.0 % (39 of 78) | 50.0 % (39 of 78) |
1. Pf1 phage coat protein
GVIDTSAVES AITDGQGDMK AIGGYIVGAL VILAVAGLIY SMLRKATemperature 297.5 (±12.5) K, pH 8.4, Details Precipitated, hydrated. The sample weight is 7-10 mg.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Pf1 phage | [U-13C; U-15N] | 7.0 ~ 10.0 mg |
Temperature 297.5 (±12.5) K, pH 8.4, Details Precipitated, hydrated. The sample weight is 7-10 mg. The sample is prepared with glucose labeled only on carbon-1 position, and it results in labeling of most but not all carbonyls.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
2 | Pf1 phage | [U-15N; 13C C1-Glucose] | 7.0 ~ 10.0 mg |
Polymer type: polypeptide(L)
Total | 13C | 15N | |
---|---|---|---|
All | 36.8 % (267 of 726) | 36.4 % (213 of 585) | 38.3 % (54 of 141) |
Backbone | 39.0 % (207 of 531) | 39.2 % (154 of 393) | 38.4 % (53 of 138) |
Sidechain | 34.3 % (107 of 312) | 34.3 % (106 of 309) | 33.3 % (1 of 3) |
Aromatic | 8.3 % (2 of 24) | 8.3 % (2 of 24) | |
Methyl | 36.8 % (43 of 117) | 36.8 % (43 of 117) |
1. Pf1 phage coat protein
GVIDTSAVES AITDGQGDMK AIGGYIVGAL VILAVAGLIY SMLRKATemperature 297.5 (±12.5) K, pH 8.4, Details Precipitated, hydrated. The sample weight is 7-10 mg.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Pf1 phage | [U-13C; U-15N] | 7.0 ~ 10.0 mg |
Temperature 297.5 (±12.5) K, pH 8.4, Details Precipitated, hydrated. The sample weight is 7-10 mg. The sample is prepared with glucose labeled only on carbon-1 position, and it results in labeling of most but not all carbonyls.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
2 | Pf1 phage | [U-15N; 13C C1-Glucose] | 7.0 ~ 10.0 mg |
Polymer type: polypeptide(L)
Total | 13C | 15N | |
---|---|---|---|
All | 31.6 % (306 of 968) | 31.5 % (246 of 780) | 31.9 % (60 of 188) |
Backbone | 32.9 % (233 of 708) | 33.2 % (174 of 524) | 32.1 % (59 of 184) |
Sidechain | 30.5 % (127 of 416) | 30.6 % (126 of 412) | 25.0 % (1 of 4) |
Aromatic | 6.3 % (2 of 32) | 6.3 % (2 of 32) | |
Methyl | 33.3 % (52 of 156) | 33.3 % (52 of 156) |
1. Pf1 phage coat protein
GVIDTSAVES AITDGQGDMK AIGGYIVGAL VILAVAGLIY SMLRKATemperature 297.5 (±12.5) K, pH 8.4, Details Precipitated, hydrated. The sample weight is 7-10 mg.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Pf1 phage | [U-13C; U-15N] | 7.0 ~ 10.0 mg |
Temperature 297.5 (±12.5) K, pH 8.4, Details Precipitated, hydrated. The sample weight is 7-10 mg. The sample is prepared with glucose labeled only on carbon-1 position, and it results in labeling of most but not all carbonyls.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
2 | Pf1 phage | [U-15N; 13C C1-Glucose] | 7.0 ~ 10.0 mg |