BMRB: 15183

Backbone and side-chain methyl order parameters and effective correlation times for calmodulin in complex with CaMKKalpha peptide

Authors

Marlow, M.S., Wand, J.

Assembly

CaM/CaMKKap

Entity

1. CaMKKalpha (polymer, Thiol state: not present), 28 monomers, 3162.790 Da Detail

GSVKLIPSWT TVILVKSMLR KRSFGNPF

2. calmodulin (polymer, Thiol state: not present), 148 monomers, 16706.21 Da Detail

ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAK

Total weight

19869.0 Da

Max. entity weight

16706.21 Da

Source organism

Gallus gallus

Exptl. method

solution NMR

Data set

order_parameters

Order parameters

205 S² values in 2 lists
Pressure 1 atm, Temperature 308 K, pH 6.5 Detail

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Pressure 1 atm, Temperature 308 K, pH 6.5

Experiment name 2D 1H-15N HSQC, 2D 1H-13C HSQC

List #1 Backbone_amide_order_parmaeters, Details NMR derived backbone N-H bond vector model-free squared generalized order parameters for CaM in complex with CaMKKalphap determined with relaxation data obtained at 500 and 750 MHz (1H).

List #2 Side-chain_methyl_order_parameters, Details NMR derived side-chain model-free squared generalized order parameters for the symmetry axis of methyl groups of CaM in complex with CaMKK p determined with relaxation data obtained at 500 and 750 MHz (1H).

Release date

2007-10-04

Citation 1

Conformational dynamics of calmodulin in complex with the calmodulin-dependent kinase kinase alpha calmodulin-binding domain
Marlow, M.S., Wand, A.
Biochemistry (2006), 45, 8732-8741, PubMed 16846216 , DOI 10.1021/bi060420m , Abstract

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Citation 2

Conformational entropy in molecular recognition by proteins
Frederick, K.K., Marlow, M.S., Valentine, K.G., Wand, A.
Nature (2007), 448, 325-329, PubMed 17637663 , DOI 10.1038/nature05959 , Abstract

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Entries sharing articles BMRB: 6 entries Detail

Related entities 1. CaMKKalpha, : 1 : 3 : 17 entities Detail

Related entities 2. calmodulin, : 11 : 88 entities Detail

Interaction partners 1. CaMKKalpha, : 4 interactors Detail

Experiments performed 4 experiments Detail