BMRBj - BMREntryMaster

Found 1 Document (0.684 seconds)

BMRB: 15185

2N8J

backbone and side chain methly order parameters and correlation times for calmodulin in complex with the eNOS peptide

Authors: Frederick, K.K., Marlow, M.S., Valentine, K.G., Wand, J.
Assembly: CaM/eNOSp
Entity: 1. calmodulin (polymer, Thiol state: not present), 148 monomers, 16706.21 Da Detail
ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAK

2. eNOSp (polymer, Thiol state: not present), 20 monomers, 2178.595 Da Detail
RKKTFKEVAN AVKISASLMG
Total weight: 18884.807 Da
Max. entity weight: 16706.21 Da
Source organism: Gallus gallus
Exptl. method: solution NMR
Data set: order_parameters
Order parameters: 195 S² values in 2 lists
Pressure 1 atm, Temperature 308 K, pH 6.5 Detail
Pressure 1 atm, Temperature 308 K, pH 6.5
Experiment name 2D 1H-15N HSQC, 2D 1H-13C HSQC
List #1 backbone_order_parameters, Details NMR derived backbone N-H bond vector model-free squared generalized order parameters for CaM in complex with eNOSp determined at 500 and 600 MHz (1H)
List #2 Methyl_order_parameters, Details NMR derived side-chain model-free squared generalized order parameters for the symmetry axis of methyl groups of CaM in complex with eNOSp determined with relaxation data obtained at 500 and 600 MHz (1H).
Release date: 2007-10-04
Citation 1: Conformational entropy in molecular recognition by proteins
Frederick, K.K., Marlow, M.S., Valentine, K.G., Wand, J.
Nature (2007), 448, 325-329, PubMed 17637663 , DOI 10.1038/nature05959 , Abstract
Citation 2: Characterization of the backbone and side chain dynamics of the CaM:eNOSp complex reveals microscopic contributions to protein conformational entropy
Frederick, K.K., Warischalk, N., Valentine, K.G., Wand, J.
Biochemistry

Entries sharing articles BMRB: 6 entries Detail

  BMRB: 15191 released on 2015-07-22
    Title backbone and sidechain order parameters for CaM-nNOSp
  BMRB: 15183 released on 2007-10-04
    Title Backbone and side-chain methyl order parameters and effective correlation times for calmodulin in complex with CaMKKalpha peptide
  BMRB: 15184 released on 2007-10-04
    Title backbone and side chain methly order parameters and correlation times for calmodulin in complex with the eNOS peptide
  BMRB: 15186 released on 2007-10-04
    Title backbone and side chain methly order parameters and correlation times for calmodulin in complex with the smMLCK peptide
  BMRB: 15187 released on 2007-10-04
    Title Backbone and side-chain methyl order parameters and effective correlation times for calmodulin in complex with Phosphodiesterase1A peptide
  BMRB: 15188 released on 2007-10-04
    Title backbone and side chain methly order parameters and correlation times for calcium-saturated calmodulin

#	Name	Isotope labeling	Concentration
1	calmodulin	[U-100% 15N]	1 mM
2	eNOSp	natural abundance	1.2 (±0.1) mM
3	potassium chloride	natural abundance	100 mM
4	imidazole	natural abundance	20 mM
5	CaCl2	natural abundance	6 mM
6	sodium azide	natural abundance	3 mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

#	Name	Isotope labeling	Concentration
1	calmodulin	[U-100% 15N]	1 mM
2	eNOSp	natural abundance	1.2 (±0.1) mM
3	potassium chloride	natural abundance	100 mM
4	imidazole	natural abundance	20 mM
5	CaCl2	natural abundance	6 mM
6	sodium azide	natural abundance	3 mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

#	Name	Isotope labeling	Concentration
9	calmodulin	[U-100% 13C; U-100% 15N; 55% 2H]	1 mM
10	eNOSp	natural abundance	1.2 (±0.1) mM
11	potassium chloride	natural abundance	100 mM
12	imidazole	natural abundance	20 mM
13	CaCl2	natural abundance	6 mM
14	sodium azide	natural abundance	3 mM
15	H2O	natural abundance	95 %
16	D2O	natural abundance	5 %

#	Name	Isotope labeling	Concentration
9	calmodulin	[U-100% 13C; U-100% 15N; 55% 2H]	1 mM
10	eNOSp	natural abundance	1.2 (±0.1) mM
11	potassium chloride	natural abundance	100 mM
12	imidazole	natural abundance	20 mM
13	CaCl2	natural abundance	6 mM
14	sodium azide	natural abundance	3 mM
15	H2O	natural abundance	95 %
16	D2O	natural abundance	5 %

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Found 1 Document (0.684 seconds)

BMRB: 15185

Entries sharing articles BMRB: 6 entries Detail

Related entities 1. calmodulin, : 11 : 88 entities Detail

Related entities 2. eNOSp, : 1 : 9 : 2 : 10 entities Detail

Interaction partners 2. eNOSp, : 2 interactors Detail

Experiments performed 4 experiments Detail

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