BMRB: 15186

backbone and side chain methly order parameters and correlation times for calmodulin in complex with the smMLCK peptide

Authors

Frederick, K.K., Kranz, J.K., Wand, J.

Assembly

CaM/smMLCKp

Entity

1. calmodulin (polymer, Thiol state: not present), 148 monomers, 16706.21 Da Detail

ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAK

2. smMLCKp (polymer, Thiol state: not present), 21 monomers, 2335.673 Da Detail

GSARRKWQKT GHAVRAIGRL S

Total weight

19041.885 Da

Max. entity weight

16706.21 Da

Source organism

Gallus gallus

Exptl. method

solution NMR

Data set

order_parameters

Order parameters

175 S² values in 2 lists
Pressure 1 atm, Temperature 308 K, pH 6.5 Detail

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Pressure 1 atm, Temperature 308 K, pH 6.5

Experiment name 2D 1H-15N HSQC, 2D 1H-13C HSQC

List #1 backbone_order_parameters, Details NMR derived backbone N-H bond vector model-free squared generalized order parameters for CaM in complex with smMLCKp determined at 500 and 600 MHz (1H)

List #2 methyl_order_parameters, Details NMR derived side-chain model-free squared generalized order parameters for the symmetry axis of methyl groups of CaM in complex with smMLCKp determined with relaxation data obtained at 500 and 600 MHz (1H).

Release date

2007-10-04

Citation 1

Conformational entropy in molecular recognition by proteins
Frederick, K.K., Marlow, M.S., Valentine, K.G., Wand, J.
Nature (2007), 448, 325-329, PubMed 17637663 , DOI 10.1038/nature05959 , Abstract

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Citation 2

Characterization of the backbone and side chain dynamics of the CaM-CaMKIp complex reveals microscopic contributions to protein conformational entropy
Frederick, K.K., Kranz, J.K., Wand, J.
Biochemistry (2006), 45, 9841-9848, PubMed 16893184 , DOI 10.1021/bi060865a , Abstract

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Entries sharing articles BMRB: 6 entries Detail

Related entities 1. calmodulin, : 11 : 88 entities Detail

Related entities 2. smMLCKp, : 1 : 7 : 40 entities Detail

Interaction partners 2. smMLCKp, : 3 interactors Detail

Experiments performed 4 experiments Detail