BMRB: 15187

Backbone and side-chain methyl order parameters and effective correlation times for calmodulin in complex with Phosphodiesterase1A peptide

Authors

Marlow, M.S., Wand, A.

Assembly

CaM/PDEs

Entity

1. calmodulin (polymer, Thiol state: not present), 148 monomers, 16706.21 Da Detail

ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAK

2. PDEs (polymer, Thiol state: not present), 22 monomers, 2600.089 Da Detail

GSQTEKMWQR LKGILRSLVK QL

Total weight

19306.3 Da

Max. entity weight

16706.21 Da

Source organism

Gallus gallus

Exptl. method

solution NMR

Data set

order_parameters

Order parameters

185 S² values in 2 lists
Pressure 1 atm, Temperature 308 K, pH 6.5 Detail

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Pressure 1 atm, Temperature 308 K, pH 6.5

Experiment name 2D 1H-15N HSQC, 2D 1H-13C HSQC

List #1 Backbone_amide_order_parmaeters, Details NMR derived backbone N-H bond vector model-free squared generalized order parameters for CaM in complex with CaMKKalphap determined with relaxation data obtained at 500 and 750 MHz (1H).

List #2 side-chain_methyl_order_parameters, Details NMR derived side-chain model-free squared generalized order parameters for the symmetry axis of methyl groups of CaM in complex

Release date

2007-10-04

Citation 1

Conformational dynamics of calmodulin in complex with the phosphodiesterase1A calmodulin-binding domain
Marlow, M.S., Wand, A.
Biochemistry

Show more 1 citaion

Citation 2

Conformational entropy in molecular recognition by proteins
Frederick, K.K., Marlow, M.S., Valentine, K.G., Wand, A.
Nature (2007), 448, 325-329, PubMed 17637663 , DOI 10.1038/nature05959 , Abstract

Hide non-primary 1 citaion

Entries sharing articles BMRB: 6 entries Detail

Related entities 1. calmodulin, : 11 : 88 entities Detail

Related entities 2. PDEs, : 5 : 13 entities Detail

Interaction partners 2. PDEs, : 2 interactors Detail

Experiments performed 4 experiments Detail