BMRBj - BMREntryMaster

Found 1 Document (0.575 seconds)

BMRB: 15278

2JQG

Leader Protease

Authors

Cencic, R., Mayer, C., Juliano, M.A., Juliano, L., Konrat, R., Kontaxis, G., Skern, T.

Assembly

sLbpro

Entity

1. sLbpro (polymer, Thiol state: all free), 167 monomers, 19007.09 Da Detail

MELTLYNGEK KTFYSRPNNH DNAWLNAILQ LFRYVEEPFF DWVYSSPENL TLEAIKQLED LTGLELHEGG PPALVIWNIK HLLHTGIGTA SRPSEVCVVD GTDMCLADFH AGIFLKGQEH AVFACVTSNG WYAIDDEDFY PWTPDPSDVL VFVPYDQEPL NGEWKAK

Formula weight

19007.09 Da

Source organism

Foot-and-mouth disease virus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.6 %, Completeness: 46.4 %, Completeness (bb): 81.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	46.4 % (914 of 1968)	25.5 % (258 of 1011)	64.5 % (504 of 782)	86.9 % (152 of 175)
Backbone	81.7 % (801 of 980)	52.7 % (176 of 334)	96.7 % (474 of 490)	96.8 % (151 of 156)
Sidechain	23.2 % (265 of 1144)	12.3 % (83 of 677)	40.4 % (181 of 448)	5.3 % (1 of 19)
Aromatic	3.3 % (8 of 242)	3.3 % (4 of 121)	3.5 % (4 of 115)	0.0 % (0 of 6)
Methyl	23.9 % (44 of 184)	19.6 % (18 of 92)	28.3 % (26 of 92)

1. sLbpro

MELTLYNGEK KTFYSRPNNH DNAWLNAILQ LFRYVEEPFF DWVYSSPENL TLEAIKQLED LTGLELHEGG PPALVIWNIK HLLHTGIGTA SRPSEVCVVD GTDMCLADFH AGIFLKGQEH AVFACVTSNG WYAIDDEDFY PWTPDPSDVL VFVPYDQEPL NGEWKAK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	sLbpro	[U-100% 13C; U-100% 15N]	1 mM
2	DTT	natural abundance	5 mM
3	sodium azide	natural abundance	10 mM
4	sodium phosphate	natural abundance	20 mM
5	sodium chloride	natural abundance	50 mM
6	H2O	natural abundance	90 %
7	D2O		10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
8	sLbpro	[U-100% 15N]	1 mM
9	DTT	natural abundance	5 mM
10	sodium azide	natural abundance	10 mM
11	sodium phosphate	natural abundance	20 mM
12	sodium chloride	natural abundance	50 mM
13	H2O	natural abundance	90 %
14	D2O		10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
15	sLbpro	[U-100% 15N]	1 mM
16	DTT	natural abundance	5 mM
17	sodium azide	natural abundance	10 mM
18	sodium phosphate	natural abundance	20 mM
19	sodium chloride	natural abundance	50 mM
20	Pf1 phage	natural abundance	17 mg/mL
21	H2O	natural abundance	90 %
22	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: 0.27 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 5 models in PDB: 2JQG, Strand ID: R Detail

Release date

2007-07-05

Citation

Investigating the substrate specificity and oligomerisation of the leader protease of foot and mouth disease virus using NMR
Cencic, R., Mayer, C., Juliano, M.A., Juliano, L., Konrat, R., Kontaxis, G., Skern, T.
J. Mol. Biol. (2007), 373, 1071-1087, PubMed 17897674 , DOI 10.1016/j.jmb.2007.08.061 , Abstract

Related entities 1. sLbpro, : 1 : 2 : 3 : 9 entities Detail

Interaction partners 1. sLbpro, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC