BMRBj - BMREntryMaster

Found 1 Document (5.43 seconds)

BMRB: 15315

2Z4F

Resonance assignments for the discoidin domain of DDR2.

Authors

Ichikawa, O., Osawa, M., Nishida, N., Goshima, N., Nomura, N., Shimada, I.

Assembly

DDR2-DS

Entity

1. DDR2-DS (polymer, Thiol state: all disulfide bound), 161 monomers, 18151.26 Da Detail

NPAICRYPLG MSGGQIPDED ITASSQWSES TAAKYGRLDS EEGDGAWCPE IPVEPDDLKE FLQIDLHTLH FITLVGTQGR HAGGHGIEFA PMYKINYSRD GTRWISWRNR HGKQVLDGNS NPYDIFLKDL EPPIVARFVR FIPVTDHSMN VCMRVELYGC V

Formula weight

18151.26 Da

Entity Connection

disulfide 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS5:SG	1:CYS160:SG
2	disulfide	sing	1:CYS48:SG	1:CYS152:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 91.6 %, Completeness (bb): 98.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.6 % (1703 of 1859)	93.8 % (906 of 966)	87.4 % (636 of 728)	97.6 % (161 of 165)
Backbone	98.4 % (929 of 944)	98.8 % (322 of 326)	97.9 % (458 of 468)	99.3 % (149 of 150)
Sidechain	86.6 % (919 of 1061)	91.3 % (584 of 640)	79.6 % (323 of 406)	80.0 % (12 of 15)
Aromatic	41.1 % (74 of 180)	63.3 % (57 of 90)	15.1 % (13 of 86)	100.0 % (4 of 4)
Methyl	100.0 % (158 of 158)	100.0 % (79 of 79)	100.0 % (79 of 79)

1. DDR2-DS

NPAICRYPLG MSGGQIPDED ITASSQWSES TAAKYGRLDS EEGDGAWCPE IPVEPDDLKE FLQIDLHTLH FITLVGTQGR HAGGHGIEFA PMYKINYSRD GTRWISWRNR HGKQVLDGNS NPYDIFLKDL EPPIVARFVR FIPVTDHSMN VCMRVELYGC V

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	DDR2-DS	[U-98% 13C; U-98% 15N]	0.4 mM
2	PHOSHATE BUFFER	natural abundance	10 mM
3	NACL	natural abundance	100 mM
4	H2O	natural abundance	95 %
5	D2O		5 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	DDR2-DS	[U-98% 13C; U-98% 15N]	0.4 mM
7	PHOSHATE BUFFER	natural abundance	10 mM
8	NACL	natural abundance	100 mM
9	D2O		5 %

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
10	DDR2-DS	[U-98% 15N]	0.4 mM
11	PHOSHATE BUFFER	natural abundance	10 mM
12	NACL	natural abundance	100 mM
13	H2O	natural abundance	95 %
14	D2O		5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 2.66 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 2.66 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 3.32 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2Z4F, Strand ID: A Detail

Release date

2008-02-28

Citation

Structural basis of the collagen-binding mode of discoidin domain receptor 2
Ichikawa, O., Osawa, M., Nishida, N., Goshima, N., Nomura, N., Shimada, I.
EMBO J. (2007), 26, 4168-4176, PubMed 17703188 , DOI 10.1038/sj.emboj.7601833 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: Q16832 released on 1997-11-01
Title DDR2_HUMAN Entity Discoidin domain-containing receptor 2

Related entities 1. DDR2-DS, : 1 : 4 : 323 entities Detail

Interaction partners 1. DDR2-DS, : 9 interactors Detail

More details for 9 interactors identified by 7 citations

Experiments performed 19 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	DDR2-DS	[U-98% 13C; U-98% 15N]	0.4 mM
2	PHOSHATE BUFFER	natural abundance	10 mM
3	NACL	natural abundance	100 mM
4	H2O	natural abundance	95 %
5	D2O		5 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	DDR2-DS	[U-98% 13C; U-98% 15N]	0.4 mM
2	PHOSHATE BUFFER	natural abundance	10 mM
3	NACL	natural abundance	100 mM
4	H2O	natural abundance	95 %
5	D2O		5 %

3 2D 1H-1H NOESY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	DDR2-DS	[U-98% 13C; U-98% 15N]	0.4 mM
7	PHOSHATE BUFFER	natural abundance	10 mM
8	NACL	natural abundance	100 mM
9	D2O		5 %

4 3D CBCA(CO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	DDR2-DS	[U-98% 13C; U-98% 15N]	0.4 mM
2	PHOSHATE BUFFER	natural abundance	10 mM
3	NACL	natural abundance	100 mM
4	H2O	natural abundance	95 %
5	D2O		5 %

5 3D C(CO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	DDR2-DS	[U-98% 13C; U-98% 15N]	0.4 mM
2	PHOSHATE BUFFER	natural abundance	10 mM
3	NACL	natural abundance	100 mM
4	H2O	natural abundance	95 %
5	D2O		5 %

6 3D HNCO

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	DDR2-DS	[U-98% 13C; U-98% 15N]	0.4 mM
2	PHOSHATE BUFFER	natural abundance	10 mM
3	NACL	natural abundance	100 mM
4	H2O	natural abundance	95 %
5	D2O		5 %

7 3D HNCA

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	DDR2-DS	[U-98% 13C; U-98% 15N]	0.4 mM
2	PHOSHATE BUFFER	natural abundance	10 mM
3	NACL	natural abundance	100 mM
4	H2O	natural abundance	95 %
5	D2O		5 %

8 3D HNCACB

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	DDR2-DS	[U-98% 13C; U-98% 15N]	0.4 mM
2	PHOSHATE BUFFER	natural abundance	10 mM
3	NACL	natural abundance	100 mM
4	H2O	natural abundance	95 %
5	D2O		5 %

9 3D HBHA(CO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	DDR2-DS	[U-98% 13C; U-98% 15N]	0.4 mM
2	PHOSHATE BUFFER	natural abundance	10 mM
3	NACL	natural abundance	100 mM
4	H2O	natural abundance	95 %
5	D2O		5 %

10 3D HN(CO)CA

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	DDR2-DS	[U-98% 13C; U-98% 15N]	0.4 mM
2	PHOSHATE BUFFER	natural abundance	10 mM
3	NACL	natural abundance	100 mM
4	H2O	natural abundance	95 %
5	D2O		5 %

11 3D H(CCO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	DDR2-DS	[U-98% 13C; U-98% 15N]	0.4 mM
2	PHOSHATE BUFFER	natural abundance	10 mM
3	NACL	natural abundance	100 mM
4	H2O	natural abundance	95 %
5	D2O		5 %

12 3D HCCH-TOCSY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	DDR2-DS	[U-98% 13C; U-98% 15N]	0.4 mM
2	PHOSHATE BUFFER	natural abundance	10 mM
3	NACL	natural abundance	100 mM
4	H2O	natural abundance	95 %
5	D2O		5 %

13 3D HCCH-TOCSY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	DDR2-DS	[U-98% 13C; U-98% 15N]	0.4 mM
7	PHOSHATE BUFFER	natural abundance	10 mM
8	NACL	natural abundance	100 mM
9	D2O		5 %

14 3D HNHA

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
10	DDR2-DS	[U-98% 15N]	0.4 mM
11	PHOSHATE BUFFER	natural abundance	10 mM
12	NACL	natural abundance	100 mM
13	H2O	natural abundance	95 %
14	D2O		5 %

15 3D 1H-15N NOESY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
10	DDR2-DS	[U-98% 15N]	0.4 mM
11	PHOSHATE BUFFER	natural abundance	10 mM
12	NACL	natural abundance	100 mM
13	H2O	natural abundance	95 %
14	D2O		5 %

16 3D 1H-15N TOCSY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
10	DDR2-DS	[U-98% 15N]	0.4 mM
11	PHOSHATE BUFFER	natural abundance	10 mM
12	NACL	natural abundance	100 mM
13	H2O	natural abundance	95 %
14	D2O		5 %

17 3D 1H-13C NOESY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	DDR2-DS	[U-98% 13C; U-98% 15N]	0.4 mM
2	PHOSHATE BUFFER	natural abundance	10 mM
3	NACL	natural abundance	100 mM
4	H2O	natural abundance	95 %
5	D2O		5 %

18 3D 1H-13C NOESY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	DDR2-DS	[U-98% 13C; U-98% 15N]	0.4 mM
7	PHOSHATE BUFFER	natural abundance	10 mM
8	NACL	natural abundance	100 mM
9	D2O		5 %

19 3D HCCH-COSY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	DDR2-DS	[U-98% 13C; U-98% 15N]	0.4 mM
2	PHOSHATE BUFFER	natural abundance	10 mM
3	NACL	natural abundance	100 mM
4	H2O	natural abundance	95 %
5	D2O		5 %

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Found 1 Document (5.43 seconds)

BMRB: 15315

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. DDR2-DS, : 1 : 4 : 323 entities Detail

Interaction partners 1. DDR2-DS, : 9 interactors Detail

Experiments performed 19 experiments Detail

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