BMRBj - BMREntryMaster

Found 1 Document (0.563 seconds)

BMRB: 15316

3BAS

Electrostatic contributions to the stability of the GCN4 leucine zipper structure.

Authors

Matousek, W.M., Alexandrescu, A.T.

Assembly

GCN4

Entity

1. GCN4 (polymer, Thiol state: not reported), 33 monomers, 3828.347 Da Detail

GSMKQLEDKV EELLSKNYHL ENEVARLKKL VGE

Formula weight

3828.347 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.8 %, Completeness (bb): 98.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.8 % (358 of 403)	85.5 % (183 of 214)	93.5 % (143 of 153)	88.9 % (32 of 36)
Backbone	98.5 % (195 of 198)	98.5 % (67 of 68)	99.0 % (96 of 97)	97.0 % (32 of 33)
Sidechain	82.2 % (194 of 236)	79.5 % (116 of 146)	89.7 % (78 of 87)	0.0 % (0 of 3)
Aromatic	50.0 % (6 of 12)	100.0 % (6 of 6)	0.0 % (0 of 6)
Methyl	94.7 % (36 of 38)	100.0 % (19 of 19)	89.5 % (17 of 19)

1. GCN4p-wt

GSMKQLEDKV EELLSKNYHL ENEVARLKKL VGE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 6.2, Details 2.1mM 13C/15N GCN4p-wt, 150 mM NaCl, 10 mM phosphate buffer, pH 6.2, 25C

#	Name	Isotope labeling	Concentration
1	GCN4p-wt	[U-13C; U-15N]	2.1 mM
2	sodium chloride	natural abundance	150 mM
3	sodium phosphate	natural abundance	10 mM
4	D2O	natural abundance	10 %

Release date

2009-08-11

Citation

Electrostatic contributions to the stability of the GCN4 leucine zipper structure
Matousek, W.M., Ciani, B., Fitch, C.A., Garcia-Moreno, B.E., Kammerer, R.A., Alexandrescu, A.T.
J. Mol. Biol. (2007), 374, 206-219, PubMed 17920624 , DOI 10.1016/j.jmb.2007.09.007 , Abstract

Related entities 1. GCN4, : 1 : 5 : 58 : 75 entities Detail

Interaction partners 1. GCN4, : 13 interactors Detail

More details for 13 interactors identified by 9 citations

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr15316_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr15316_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	native_chem_shift_list_1	Warning	393		100.0 (chain: 1, length: 33)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: native_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 33, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 220
  - ¹³C chemical shifts: 142
  - ¹⁵N chemical shifts: 31
- Completeness of assignments
  - Entity_assemble_ID: 1
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	68	66	97.1
¹³C chemical shifts	66	64	97.0
¹⁵N chemical shifts	33	31	93.9

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Found 1 Document (0.563 seconds)

BMRB: 15316

Sample

Related entities 1. GCN4, : 1 : 5 : 58 : 75 entities Detail

Interaction partners 1. GCN4, : 13 interactors Detail

Experiments performed 4 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	214	182	85.0
¹³C chemical shifts	153	142	92.8
¹⁵N chemical shifts	37	31	83.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	146	116	79.5
¹³C chemical shifts	87	78	89.7
¹⁵N chemical shifts	4	0	0.0