BMRB: 15366

Human eRF1 C-domain

Authors

Mantsyzov, A.B., Polshakov, V.I., Birdsall, B., Kisselev, L.L.

Assembly

Human eRF1 C-domain

Entity

1. Human eRF1 C-domain (polymer, Thiol state: all free), 171 monomers, 19954.10 Da Detail

MSNVKFIQEK KLIGRYFDEI SQDTGKYCFG VEDTLKALEM GAVEILIVYE NLDIMRYVLH CQGTEEEKIL YLTPEQEKDK SHFTDKETGQ EHELIESMPL LEWFANNYKK FGATLEIVTD KSQEGSQFVK GFGGIGGILR YRVDFQGMEY QGGDDEFFDL DDYLEHHHHH H

Formula weight

19954.1 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 93.0 %, Completeness: 34.4 %, Completeness (bb): 62.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	34.4 % (708 of 2057)	20.2 % (216 of 1070)	42.4 % (341 of 804)	82.5 % (151 of 183)
Backbone	62.2 % (636 of 1022)	46.3 % (165 of 356)	64.4 % (320 of 497)	89.3 % (151 of 169)
Sidechain	7.2 % (86 of 1190)	7.1 % (51 of 714)	7.6 % (35 of 462)	0.0 % (0 of 14)
Aromatic	3.5 % (8 of 230)	3.5 % (4 of 115)	3.5 % (4 of 114)	0.0 % (0 of 1)
Methyl	13.8 % (22 of 160)	15.0 % (12 of 80)	12.5 % (10 of 80)

1. C-domain of human polypeptide release factor eRF1

MSNVKFIQEK KLIGRYFDEI SQDTGKYCFG VEDTLKALEM GAVEILIVYE NLDIMRYVLH CQGTEEEKIL YLTPEQEKDK SHFTDKETGQ EHELIESMPL LEWFANNYKK FGATLEIVTD KSQEGSQFVK GFGGIGGILR YRVDFQGMEY QGGDDEFFDL DDYLEHHHHH H

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	C-domain of human polypeptide release factor eRF1	natural abundance		1 mM
2	potassium phosphate	natural abundance		10 mM
3	potassium chloride	natural abundance		50 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
4	C-domain of human polypeptide release factor eRF1	[U-99% 15N]		1.1 mM
5	potassium phosphate	natural abundance		10 mM
6	potassium chloride	natural abundance		20 mM

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
7	C-domain of human polypeptide release factor eRF1	[U-99% 13C; U-99% 15N]		0.8 mM
8	potassium phosphate	natural abundance		10 mM
9	potassium chloride	natural abundance		20 mM

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Chem. Shift Complete2

Sequence coverage: 17.0 %, Completeness: 20.9 %, Completeness (bb): 36.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	20.9 % (858 of 4114)	13.8 % (295 of 2140)	24.2 % (389 of 1608)	47.5 % (174 of 366)
Backbone	36.4 % (743 of 2044)	29.4 % (209 of 712)	36.2 % (360 of 994)	51.5 % (174 of 338)
Sidechain	5.8 % (137 of 2380)	6.0 % (85 of 1428)	5.6 % (52 of 924)	0.0 % (0 of 28)
Aromatic	3.5 % (16 of 460)	3.5 % (8 of 230)	3.5 % (8 of 228)	0.0 % (0 of 2)
Methyl	9.7 % (31 of 320)	11.2 % (18 of 160)	8.1 % (13 of 160)

1. C-domain of human polypeptide release factor eRF1

MSNVKFIQEK KLIGRYFDEI SQDTGKYCFG VEDTLKALEM GAVEILIVYE NLDIMRYVLH CQGTEEEKIL YLTPEQEKDK SHFTDKETGQ EHELIESMPL LEWFANNYKK FGATLEIVTD KSQEGSQFVK GFGGIGGILR YRVDFQGMEY QGGDDEFFDL DDYLEHHHHH H

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	C-domain of human polypeptide release factor eRF1	natural abundance		1 mM
2	potassium phosphate	natural abundance		10 mM
3	potassium chloride	natural abundance		50 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
4	C-domain of human polypeptide release factor eRF1	[U-99% 15N]		1.1 mM
5	potassium phosphate	natural abundance		10 mM
6	potassium chloride	natural abundance		20 mM

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
7	C-domain of human polypeptide release factor eRF1	[U-99% 13C; U-99% 15N]		0.8 mM
8	potassium phosphate	natural abundance		10 mM
9	potassium chloride	natural abundance		20 mM

Hide sample codition

Protein Blocks Logo

Calculated from 24 models in PDB: 2KTU, Strand ID: A Detail

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Release date

2015-09-02

Citation

NMR assignments of the C-terminal domain of human polypeptide release factor eRF1
Mantsyzov, A.B., Ivanova, E.V., Birdsall, B., Kolosov, P.M., Kisselev, L.L., Polshakov, V.I.
Biomol. NMR Assign. (2007), 1, 183-185, PubMed 19636860 , DOI 10.1007/s12104-007-9050-z , Abstract

Related entities 1. Human eRF1 C-domain, : 1 : 2 : 119 entities Detail

Interaction partners 1. Human eRF1 C-domain, : 3 interactors Detail

Experiments performed 13 experiments Detail

Chemical shift validation 4 contents Detail