BMRBj - BMREntryMaster

Found 1 Document (0.458 seconds)

BMRB: 15398

2KKJ

NH, NN, C and CA chemical shift assignment of the nuclear coactivator binding domain of CBP in the free state

Authors

Ebert, M., Bae, S., Dyson, H.JANE., Wright, P.E.

Assembly

CBP

Entity

1. CBP (polymer, Thiol state: not present), 59 monomers, 6561.503 Da Detail

PNRSISPSAL QDLLRTLKSP SSPQQQQQVL NILKSNPQLM AAFIKQRTAK YVANQPGMQ

Formula weight

6561.503 Da

Source organism

Mus musculus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.8 %, Completeness: 33.1 %, Completeness (bb): 61.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	33.1 % (236 of 714)	19.1 % (73 of 382)	43.0 % (114 of 265)	73.1 % (49 of 67)
Backbone	61.1 % (209 of 342)	46.0 % (52 of 113)	61.9 % (109 of 176)	90.6 % (48 of 53)
Sidechain	7.2 % (31 of 430)	7.8 % (21 of 269)	6.1 % (9 of 147)	7.1 % (1 of 14)
Aromatic	0.0 % (0 of 18)	0.0 % (0 of 9)	0.0 % (0 of 9)
Methyl	0.0 % (0 of 62)	0.0 % (0 of 31)	0.0 % (0 of 31)

1. CBP

PNRSISPSAL QDLLRTLKSP SSPQQQQQVL NILKSNPQLM AAFIKQRTAK YVANQPGMQ

Show sample condition

Sample

Pressure 1 atm, Temperature 304.15 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
1	CBP	[U-98% 13C; U-98% 15N]		1 mM
2	NaCl			50 mM

Release date

2008-04-03

Citation

NMR relaxation study of the complex formed between CBP and the activation domain of the nuclear hormone receptor coactivator ACTR
Ebert, M., Bae, S., Dyson, H.JANE., Wright, P.E.
Biochemistry (2008), 47, 1299-1308, PubMed 18177052 , DOI 10.1021/bi701767j , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 15397 released on 2008-04-03
Title NH, NN, C, CA and CB chemical shift assignment of the transcriptional activation domain 1 of the nuclear hormone receptor coactivator ACTR in the free state

Related entities 1. CBP, : 1 : 3 : 8 : 22 entities Detail

Interaction partners 1. CBP, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 6 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr15398_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr15398_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	NH_NN_C_and_CA_chemical_shifts	Warning	201		89.8 (chain: 1, length: 59)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: NH_NN_C_and_CA_chemical_shifts
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 59, Sequence coverage: 89.8%
- Total number of assignments
  - ¹H chemical shifts: 48
  - ¹³C chemical shifts: 105
  - ¹⁵N chemical shifts: 48
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	382	48	12.6
¹³C chemical shifts	265	105	39.6
¹⁵N chemical shifts	70	48	68.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	113	48	42.5
¹³C chemical shifts	118	105	89.0
¹⁵N chemical shifts	53	48	90.6

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Found 1 Document (0.458 seconds)

BMRB: 15398

Sample

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. CBP, : 1 : 3 : 8 : 22 entities Detail

Interaction partners 1. CBP, : 1 interactors Detail

Experiments performed 6 experiments Detail

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Chemical shift validation 3 contents Detail