BMRBj - BMREntryMaster

Found 1 Document (0.515 seconds)

BMRB: 15424

2KB0

NMR Assignment of Cdc42(T35A), and Active Switch I mutant of Cdc42

Authors

Oswald, R.E.

Assembly

Cdc42(T35A) monomer

Entity

1. Cdc42(T35A) monomer (polymer, Thiol state: not reported), 178 monomers, 19723.47 Da Detail

MQTIKCVVVG DGAVGKTCLL ISYTTNKFPS EYVPAVFDNY AVTVMIGGEP YTLGLFDTAG QEDYDRLRPL SYPQTDVFLV CFSVVSPSSF ENVKEKWVPE ITHHCPKTPF LLVGTQIDLR DDPSTIEKLA KNKQKPITPE TAEKLARDLK AVKYVECSAL TQKGLKNVFD EAILAALE

Formula weight

19723.47 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.4 %, Completeness: 75.2 %, Completeness (bb): 96.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	75.2 % (1562 of 2077)	67.8 % (727 of 1072)	80.0 % (662 of 827)	97.2 % (173 of 178)
Backbone	96.4 % (1006 of 1044)	96.6 % (341 of 353)	95.6 % (502 of 525)	98.2 % (163 of 166)
Sidechain	59.7 % (718 of 1202)	53.7 % (386 of 719)	68.4 % (322 of 471)	83.3 % (10 of 12)
Aromatic	7.1 % (11 of 156)	12.8 % (10 of 78)	0.0 % (0 of 77)	100.0 % (1 of 1)
Methyl	71.2 % (161 of 226)	73.5 % (83 of 113)	69.0 % (78 of 113)

1. Cdc42(T35A)

MQTIKCVVVG DGAVGKTCLL ISYTTNKFPS EYVPAVFDNY AVTVMIGGEP YTLGLFDTAG QEDYDRLRPL SYPQTDVFLV CFSVVSPSSF ENVKEKWVPE ITHHCPKTPF LLVGTQIDLR DDPSTIEKLA KNKQKPITPE TAEKLARDLK AVKYVECSAL TQKGLKNVFD EAILAALE

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
1	Cdc42(T35A)	[U-100% 13C; U-100% 15N]		0.3 ~ 0.5 mM

Release date

2007-08-15

Citation

NMR assignment of Cdc42(T35A), an active Switch I mutant of Cdc42
Adams, P.D., Oswald, R.E.
Biomol. NMR Assign. (2007), 1, 225-227, PubMed 19636871 , DOI 10.1007/s12104-007-9062-8 , Abstract

Related entities 1. Cdc42(T35A) monomer, : 1 : 43 : 257 entities Detail

Interaction partners 1. Cdc42(T35A) monomer, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 1 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr15424_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr15424_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1667		99.4 (chain: 1, length: 178)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 178, Sequence coverage: 99.4%
- Total number of assignments
  - ¹H chemical shifts: 852
  - ¹³C chemical shifts: 643
  - ¹⁵N chemical shifts: 172
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	1072	700	65.3
¹³C chemical shifts	827	643	77.8
¹⁵N chemical shifts	182	172	94.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	353	342	96.9
¹³C chemical shifts	356	336	94.4
¹⁵N chemical shifts	166	163	98.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	719	358	49.8
¹³C chemical shifts	471	307	65.2
¹⁵N chemical shifts	16	9	56.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	115	76	66.1
¹³C chemical shifts	115	70	60.9

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Found 1 Document (0.515 seconds)

BMRB: 15424

Sample

Related entities 1. Cdc42(T35A) monomer, : 1 : 43 : 257 entities Detail

Interaction partners 1. Cdc42(T35A) monomer, : 1 interactors Detail

Experiments performed 1 experiments Detail

Instrument

Sample

Chemical shift validation 3 contents Detail

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	78	10	12.8
¹³C chemical shifts	77	0	0.0
¹⁵N chemical shifts	1	1	100.0