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BMRB: 15476

2JVD

Solution NMR structure of the folded N-terminal fragment of UPF0291 protein ynzC from Bacillus subtilis. Northeast Structural Genomics target SR384-1-46.

Authors

Aramini, J.M., Sharma, S., Huang, Y.J., Zhao, L., Owens, L.A., Stokes, K., Jiang, M., Xiao, R., Baran, M.C., Swapna, G.V.T., Acton, T.B., Montelione, G.T.

Assembly

SR384-1-46

Entity

1. SR384-1-46 (polymer, Thiol state: not present), 54 monomers, 6274.111 Da Detail

MISNAKIARI NELAAKAKAG VITEEEKAEQ QKLRQEYLKG FRSSMKLEHH HHHH

Formula weight

6274.111 Da

Source organism

Bacillus subtilis

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.9 %, Completeness: 87.2 %, Completeness (bb): 85.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.2 % (570 of 654)	87.9 % (304 of 346)	86.7 % (216 of 249)	84.7 % (50 of 59)
Backbone	85.2 % (276 of 324)	84.5 % (93 of 110)	86.3 % (138 of 160)	83.3 % (45 of 54)
Sidechain	86.9 % (332 of 382)	87.7 % (207 of 236)	85.1 % (120 of 141)	100.0 % (5 of 5)
Aromatic	42.9 % (18 of 42)	42.9 % (9 of 21)	42.9 % (9 of 21)
Methyl	100.0 % (52 of 52)	100.0 % (26 of 26)	100.0 % (26 of 26)

1. SR384-1-46

MISNAKIARI NELAAKAKAG VITEEEKAEQ QKLRQEYLKG FRSSMKLEHH HHHH

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	SR384-1-46	[U-100% 13C; U-100% 15N]	1.36 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	10 mM
5	calcium chloride	natural abundance	5 mM
6	sodium azide	natural abundance	0.02 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	SR384-1-46	[U-5% 13C; U-100% 15N]	1.1 mM
8	MES	natural abundance	20 mM
9	sodium chloride	natural abundance	100 mM
10	DTT	natural abundance	10 mM
11	calcium chloride	natural abundance	5 mM
12	sodium azide	natural abundance	0.02 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2JVD, Strand ID: A Detail

Release date

2007-09-20

Citation

Solution NMR structure of the SOS response protein YnzC from Bacillus subtilis
Aramini, J.M., Sharma, S., Huang, Y.J., Swapna, G.V.T., Ho, C., Shetty, K., Cunningham, K., Ma, L., Zhao, L., Owens, L.A., Jiang, M., Xiao, R., Liu, J., Baran, M.C., Acton, T.B., Rost, B., Montelione, G.T.
Proteins (2008), 72, 526-530, PubMed 18431750 , DOI 10.1002/prot.22064 , Abstract

Related entities 1. SR384-1-46, : 1 : 2 : 50 entities Detail

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC