BMRB: 15480

1H assignments of W60G mutant of human beta2-microglobulin

Authors

Esposito, G., Corazza, A., Rennella, E., Gumral, D., Mimmi, M., Fogolari, F., Viglino, P., Raimondi, S., Giorgetti, S., Bolognesi, B., Merlini, G., Stoppini, M., Bellotti, V.

Assembly

w60g-b2m

Entity

1. w60g-b2m (polymer, Thiol state: all disulfide bound), 100 monomers, 11733.06 Da Detail

MIQRTPKIQV YSRHPAENGK SNFLNCYVSG FHPSDIEVDL LKNGERIEKV EHSDLSFSKD GSFYLLYYTE FTPTEKDEYA CRVNHVTLSQ PKIVKWDRDM

Formula weight

11733.06 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS26:SG	1:CYS81:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.4 %, Completeness (bb): 98.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	95.4 % (603 of 632)	95.4 % (603 of 632)
Backbone	98.5 % (196 of 199)	98.5 % (196 of 199)
Sidechain	94.0 % (407 of 433)	94.0 % (407 of 433)
Aromatic	100.0 % (63 of 63)	100.0 % (63 of 63)
Methyl	97.8 % (44 of 45)	97.8 % (44 of 45)

1. w60g-b2m

MIQRTPKIQV YSRHPAENGK SNFLNCYVSG FHPSDIEVDL LKNGERIEKV EHSDLSFSKD GSFYLLYYTE FTPTEKDEYA CRVNHVTLSQ PKIVKWDRDM

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Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.6, Details 70 mM phosphate, 100 mM NaCl

#	Name	Isotope labeling	Type	Concentration
1	w60g-b2m	natural abundance		0.5-0.9 mM
2	phosphate			70 mM
3	NaCl			100 mM
4	H2O			95 %
5	D2O			5 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2VB5, Strand ID: A Detail

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Release date

2007-10-14

Citation

The controlling roles of Trp60 and Trp95 in beta2-microglobulin function, folding and amyloid aggregation properties
Esposito, G., Ricagno, S., Corazza, A., Rennella, E., Gumral, D., Mimmi, M., Betto, E., Pucillo, C., Fogolari, F., Viglino, P., Raimondi, S., Giorgetti, S., Bolognesi, B., Merlini, G., Stoppini, M., Bolognesi, M., Bellotti, V.
J. Mol. Biol. (2008), 378, 887-897, PubMed 18395224 , DOI 10.1016/j.jmb.2008.03.002 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. w60g-b2m, : 1 : 1 : 1 : 101 : 185 entities Detail

Interaction partners 1. w60g-b2m, : 121 interactors Detail

Experiments performed 3 experiments Detail