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BMRB: 15498

4Q5E

Backbone and side-chain 1H, 13C and 15N resonance assignments for the E2 conjugating enzyme, UbcH7

Authors

Serniwka, S.A., Shaw, G.S.

Assembly

UbcH7

Entity

1. UbcH7 (polymer, Thiol state: all free), 153 monomers, 17698.02 Da Detail

AASRRLMKEL EEIRKSGMKN FRNIQVDEAN LLTWQGLIVP DNPPYDKGAF RIEINFPAEY PFKPPKITFK TKIYHPNIDE KGQVCLPVIS AENWKPATKT DQVIQSLIAL VNDPQPEHPL RADLAEEYSK DRKKFSKNAE EFTKKYGEKR PVD

Formula weight

17698.02 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 73.5 %, Completeness (bb): 92.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	73.5 % (1387 of 1887)	62.7 % (629 of 1003)	83.4 % (607 of 728)	96.8 % (151 of 156)
Backbone	92.0 % (819 of 890)	83.5 % (248 of 297)	95.6 % (434 of 454)	98.6 % (137 of 139)
Sidechain	62.4 % (714 of 1145)	54.0 % (381 of 706)	75.6 % (319 of 422)	82.4 % (14 of 17)
Aromatic	16.9 % (24 of 142)	18.3 % (13 of 71)	15.9 % (11 of 69)	0.0 % (0 of 2)
Methyl	96.6 % (141 of 146)	97.3 % (71 of 73)	95.9 % (70 of 73)

1. UbcH7

AASRRLMKEL EEIRKSGMKN FRNIQVDEAN LLTWQGLIVP DNPPYDKGAF RIEINFPAEY PFKPPKITFK TKIYHPNIDE KGQVCLPVIS AENWKPATKT DQVIQSLIAL VNDPQPEHPL RADLAEEYSK DRKKFSKNAE EFTKKYGEKR PVD

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
1	UbcH7	[U-100% 13C; U-100% 15N]	0.5 mM
2	TRIS	natural abundance	25 mM
3	EDTA	natural abundance	1 mM
4	DTT	natural abundance	5 mM
5	sodium chloride	natural abundance	200 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.18 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.18 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 4 Detail

Release date

2008-01-16

Citation

1H, 13C and 15N resonance assignments for the human E2 conjugating enzyme, UbcH7
Serniwka, S.A., Shaw, G.S.
Biomol. NMR Assign. (2008), 2, 21-23, PubMed 19636915 , DOI 10.1007/s12104-007-9074-4 , Abstract

Related entities 1. UbcH7, : 1 : 2 : 2 : 18 : 268 entities Detail

Interaction partners 1. UbcH7, : 45 interactors Detail

More details for 45 interactors identified by 21 citations

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC