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BMRB: 15501

6HVO

Backbone assignment of human Proliferating Cell Nuclear Antigen.

Authors

Sanchez, R., Torres, D., Prieto, J., Blanco, F., Campos-Olivas, R.

Assembly

human PCNA trimer

Entity

1. human PCNA trimer (polymer, Thiol state: all free), 264 monomers, 29059.78 × 3 Da Detail

GPHMFEARLV QGSILKKVLE ALKDLINEAC WDISSSGVNL QSMDSSHVSL VQLTLRSEGF DTYRCDRNLA MGVNLTSMSK ILKCAGNEDI ITLRAEDNAD TLALVFEAPN QEKVSDYEMK LMDLDVEQLG IPEQEYSCVV KMPSGEFARI CRDLSHIGDA VVISCAKDGV KFSASGELGN GNIKLSQTSN VDKEEEAVTI EMNEPVQLTF ALRYLNFFTK ATPLSSTVTL SMSADVPLVV EYKIADMGHL KYYLAPKIED EEGS

Total weight

87179.336 Da

Max. entity weight

29059.78 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.1 %, Completeness: 48.8 %, Completeness (bb): 81.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	48.8 % (1461 of 2995)	26.8 % (415 of 1546)	66.9 % (784 of 1172)	94.6 % (262 of 277)
Backbone	81.3 % (1274 of 1568)	52.5 % (281 of 535)	96.5 % (750 of 777)	94.9 % (243 of 256)
Sidechain	25.5 % (428 of 1676)	13.4 % (135 of 1011)	42.5 % (274 of 644)	90.5 % (19 of 21)
Aromatic	1.2 % (2 of 164)	1.2 % (1 of 82)	0.0 % (0 of 81)	100.0 % (1 of 1)
Methyl	17.3 % (55 of 318)	14.5 % (23 of 159)	20.1 % (32 of 159)

1. human PCNA

Show sample condition

Sample #1

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	human PCNA	[U-99% 13C; U-100% 15N; U-93% 2H]	1.0 mM
2	sodium chloride	natural abundance	137.0 mM
3	potassium chloride	natural abundance	2.7 mM
4	sodium phosphate	natural abundance	10.0 mM
5	potassium phosphate	natural abundance	2.0 mM

Sample #2

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	human PCNA	[U-15N]-Leu	1.0 mM
7	sodium chloride	natural abundance	137.0 mM
8	potassium chloride	natural abundance	2.7 mM
9	sodium phosphate	natural abundance	10.0 mM
10	potassium phosphate	natural abundance	2.0 mM

Sample #3

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	human PCNA	[U-15N]-Val	1.0 mM
12	sodium chloride	natural abundance	137.0 mM
13	potassium chloride	natural abundance	2.7 mM
14	sodium phosphate	natural abundance	10.0 mM
15	potassium phosphate	natural abundance	2.0 mM

Sample #4

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
16	human PCNA	[U-15N]-Ile	1.0 mM
17	sodium chloride	natural abundance	137.0 mM
18	potassium chloride	natural abundance	2.7 mM
19	sodium phosphate	natural abundance	10.0 mM
20	potassium phosphate	natural abundance	2.0 mM

Sample #5

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0

#	Name	Isotope labeling	Concentration
21	human PCNA	[U-15N]-Phe, [U-15N]-Tyr	1.0 mM
22	sodium chloride	natural abundance	137.0 mM
23	potassium chloride	natural abundance	2.7 mM
24	sodium phosphate	natural abundance	10.0 mM
25	potassium phosphate	natural abundance	2.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.6 ppm	internal	indirect	0.2514495
¹H	water	protons	4.6 ppm	internal	direct	1.0
¹⁵N	water	protons	4.6 ppm	internal	indirect	0.1013291

Referencing offset: 0.33 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.6 ppm	internal	indirect	0.2514495
¹H	water	protons	4.6 ppm	internal	direct	1.0
¹⁵N	water	protons	4.6 ppm	internal	indirect	0.1013291

Referencing offset: 0.33 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.6 ppm	internal	indirect	0.2514495
¹H	water	protons	4.6 ppm	internal	direct	1.0
¹⁵N	water	protons	4.6 ppm	internal	indirect	0.1013291

Referencing offset: 0.66 ppm, Outliers: 1 Detail

Release date

2007-10-01

Citation

Backbone assignment of human proliferating cell nuclear antigen
Sanchez, R., Torres, D., Prieto, J., Blanco, F., Campos-Olivas, R.
Biomol. NMR Assign. (2007), 1, 245-247, PubMed 19636876 , DOI 10.1007/s12104-007-9068-2 , Abstract

Related entities 1. human PCNA trimer, : 1 : 11 : 49 : 162 entities Detail

Interaction partners 1. human PCNA trimer, : 105 interactors Detail

More details for 105 interactors identified by 51 citations