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Backbone assignment of human Proliferating Cell Nuclear Antigen.
Authors
Sanchez, R., Torres, D., Prieto, J., Blanco, F., Campos-Olivas, R.
Assembly
human PCNA trimer
Entity
1. human PCNA trimer (polymer, Thiol state: all free), 264 monomers, 29059.78 × 3 Da Detail

GPHMFEARLV QGSILKKVLE ALKDLINEAC WDISSSGVNL QSMDSSHVSL VQLTLRSEGF DTYRCDRNLA MGVNLTSMSK ILKCAGNEDI ITLRAEDNAD TLALVFEAPN QEKVSDYEMK LMDLDVEQLG IPEQEYSCVV KMPSGEFARI CRDLSHIGDA VVISCAKDGV KFSASGELGN GNIKLSQTSN VDKEEEAVTI EMNEPVQLTF ALRYLNFFTK ATPLSSTVTL SMSADVPLVV EYKIADMGHL KYYLAPKIED EEGS


Total weight
87179.336 Da
Max. entity weight
29059.78 Da
Source organism
Homo sapiens
Exptl. method
SOLUTION NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 98.9 %, Completeness: 45.9 %, Completeness (bb): 80.1 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All45.9 % (1377 of 3003)21.4 % (331 of 1546)66.7 % (782 of 1172)92.6 % (264 of 285)
Backbone80.1 % (1244 of 1553)46.3 % (241 of 520)97.7 % (759 of 777)95.3 % (244 of 256)
Sidechain22.0 % (374 of 1699) 8.8 % (90 of 1026)41.0 % (264 of 644)69.0 % (20 of 29)
Aromatic 1.2 % (2 of 164) 1.2 % (1 of 82) 0.0 % (0 of 81)100.0 % (1 of 1)
Methyl11.3 % (36 of 318) 5.7 % (9 of 159)17.0 % (27 of 159)

1. human PCNA

GPHMFEARLV QGSILKKVLE ALKDLINEAC WDISSSGVNL QSMDSSHVSL VQLTLRSEGF DTYRCDRNLA MGVNLTSMSK ILKCAGNEDI ITLRAEDNAD TLALVFEAPN QEKVSDYEMK LMDLDVEQLG IPEQEYSCVV KMPSGEFARI CRDLSHIGDA VVISCAKDGV KFSASGELGN GNIKLSQTSN VDKEEEAVTI EMNEPVQLTF ALRYLNFFTK ATPLSSTVTL SMSADVPLVV EYKIADMGHL KYYLAPKIED EEGS

Sample #1

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0


#NameIsotope labelingTypeConcentration
1human PCNA[U-99% 13C; U-100% 15N; U-93% 2H]1.0 mM
2sodium chloridenatural abundance137.0 mM
3potassium chloridenatural abundance2.7 mM
4sodium phosphatenatural abundance10.0 mM
5potassium phosphatenatural abundance2.0 mM
Sample #2

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0


#NameIsotope labelingTypeConcentration
6human PCNA[U-15N]-Leu1.0 mM
7sodium chloridenatural abundance137.0 mM
8potassium chloridenatural abundance2.7 mM
9sodium phosphatenatural abundance10.0 mM
10potassium phosphatenatural abundance2.0 mM
Sample #3

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0


#NameIsotope labelingTypeConcentration
11human PCNA[U-15N]-Val1.0 mM
12sodium chloridenatural abundance137.0 mM
13potassium chloridenatural abundance2.7 mM
14sodium phosphatenatural abundance10.0 mM
15potassium phosphatenatural abundance2.0 mM
Sample #4

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0


#NameIsotope labelingTypeConcentration
16human PCNA[U-15N]-Ile1.0 mM
17sodium chloridenatural abundance137.0 mM
18potassium chloridenatural abundance2.7 mM
19sodium phosphatenatural abundance10.0 mM
20potassium phosphatenatural abundance2.0 mM
Sample #5

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 308 K, pH 7.0


#NameIsotope labelingTypeConcentration
21human PCNA[U-15N]-Phe, [U-15N]-Tyr1.0 mM
22sodium chloridenatural abundance137.0 mM
23potassium chloridenatural abundance2.7 mM
24sodium phosphatenatural abundance10.0 mM
25potassium phosphatenatural abundance2.0 mM

LACS Plot; CA
Referencing offset: 0.33 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: 0.33 ppm, Outliers: 2 Detail
LACS Plot; CO
Referencing offset: 0.66 ppm, Outliers: 1 Detail
Release date
2008-01-27
Citation
Backbone assignment of human proliferating cell nuclear antigen
Sanchez, R., Torres, D., Prieto, J., Blanco, F., Campos-Olivas, R.
Biomol. NMR Assign. (2007), 1, 245-247, PubMed 19636876 , DOI 10.1007/s12104-007-9068-2 ,
Related entities 1. human PCNA trimer, : 1 : 2 : 35 : 156 entities Detail
Interaction partners 1. human PCNA trimer, : 101 interactors Detail
Experiments performed 11 experiments Detail