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1H, 15N, 13CO, 13Calpha and 13Cbeta Chemical Shift Assignments of Human Peroxisome Proliferator-Activated Receptor Gamma Ligand-Binding Domain Bound to GW1929
Authors
Lu, J.
Assembly
GW1929-PPARgamma Ligand-Binding Domain Complex
Entity
1. Peroxisome Proliferator-Activated Receptor Gamma Ligand-Binding Domain (polymer, Thiol state: all free), 279 monomers, 31823.63 Da Detail

GSHMQLNPES ADLRALAKHL YDSYIKSFPL TKAKARAILT GKTTDKSPFV IYDMNSLMMG EDKIKFKHIT PLQEQSKEVA IRIFQGCQFR SVEAVQEITE YAKSIPGFVN LDLNDQVTLL KYGVHEIIYT MLASLMNKDG VLISEGQGFM TREFLKSLRK PFGDFMEPKF EFAVKFNALE LDDSDLAIFI AVIILSGDRP GLLNVKPIED IQDNLLQALE LQLKLNHPES SQLFAKLLQK MTDLRQIVTE HVQLLQVIKK TETDMSLHPL LQEIYKDLY


2. GW1929 (non-polymer), na Da
Formula weight
31823.63 Da
Source organism
Homo sapiens
Exptl. method
SOLUTION NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 95.3 %, Completeness: 42.8 %, Completeness (bb): 78.9 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All42.8 % (1460 of 3409)21.8 % (386 of 1774)61.9 % (825 of 1333)82.5 % (249 of 302)
Backbone78.9 % (1294 of 1640)49.0 % (268 of 547)94.2 % (777 of 825)92.9 % (249 of 268)
Sidechain20.6 % (419 of 2036) 9.6 % (118 of 1227)38.8 % (301 of 775) 0.0 % (0 of 34)
Aromatic 0.0 % (0 of 242) 0.0 % (0 of 121) 0.0 % (0 of 121)
Methyl16.1 % (57 of 354)11.9 % (21 of 177)20.3 % (36 of 177)

1. Peroxisome Proliferator-Activated Receptor Gamma Ligand-Binding Domain

GSHMQLNPES ADLRALAKHL YDSYIKSFPL TKAKARAILT GKTTDKSPFV IYDMNSLMMG EDKIKFKHIT PLQEQSKEVA IRIFQGCQFR SVEAVQEITE YAKSIPGFVN LDLNDQVTLL KYGVHEIIYT MLASLMNKDG VLISEGQGFM TREFLKSLRK PFGDFMEPKF EFAVKFNALE LDDSDLAIFI AVIILSGDRP GLLNVKPIED IQDNLLQALE LQLKLNHPES SQLFAKLLQK MTDLRQIVTE HVQLLQVIKK TETDMSLHPL LQEIYKDLY

Sample

Pressure 1 atm, Temperature 298 K, pH 7.4


#NameIsotope labelingTypeConcentration
1Peroxisome Proliferator-Activated Receptor Gamma Ligand-Binding Domain[U-13C; U-15N; U-2H]1.0 mM
2potassium phosphatenatural abundance20 mM
3KClnatural abundance50 mM
4NaN3natural abundance0.05 %
5EDTA-d16natural abundance0.5 mM
6b-mercaptoethanol-d6natural abundance8 mM
7H20natural abundance95 %
8D2O5 %

LACS Plot; CA
Referencing offset: 0.51 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 0.51 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: -0.36 ppm, Outliers: 2 Detail
Release date
2008-01-10
Citation
Effect of heterodimer partner RXRalpha on PPARgamma activation function-2 helix in solution
Lu, J., Chen, M., Stanley, S.E., Li, E.
Biochem. Biophys. Res. Commun. (2008), 365, 42-46, PubMed 17980149 , DOI 10.1016/j.bbrc.2007.10.143 ,
Related entities 1. Peroxisome Proliferator-Activated Receptor Gamma Ligand-Binding Domain, : 1 : 1 : 33 : 88 entities Detail
Interaction partners 1. Peroxisome Proliferator-Activated Receptor Gamma Ligand-Binding Domain, : 1 interactors Detail
Experiments performed 7 experiments Detail
Keywords Allosteric communication, Heterodimerization, NMR, PPARgamma, RXRalpha