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BMRB: 15518

8HUP

1H, 15N, 13CO, 13Calpha and 13Cbeta Chemical Shift Assignments of Human Peroxisome Proliferator-Activated Receptor Gamma Ligand-Binding Domain Bound to GW1929

Authors

Lu, J.

Assembly

GW1929-PPARgamma Ligand-Binding Domain Complex

Entity

1. Peroxisome Proliferator-Activated Receptor Gamma Ligand-Binding Domain (polymer, Thiol state: all free), 279 monomers, 31823.63 Da Detail

GSHMQLNPES ADLRALAKHL YDSYIKSFPL TKAKARAILT GKTTDKSPFV IYDMNSLMMG EDKIKFKHIT PLQEQSKEVA IRIFQGCQFR SVEAVQEITE YAKSIPGFVN LDLNDQVTLL KYGVHEIIYT MLASLMNKDG VLISEGQGFM TREFLKSLRK PFGDFMEPKF EFAVKFNALE LDDSDLAIFI AVIILSGDRP GLLNVKPIED IQDNLLQALE LQLKLNHPES SQLFAKLLQK MTDLRQIVTE HVQLLQVIKK TETDMSLHPL LQEIYKDLY

2. GW1929 (non-polymer), na Da

Formula weight

31823.63 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.6 %, Completeness: 44.7 %, Completeness (bb): 80.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	44.7 % (1519 of 3401)	25.8 % (457 of 1774)	61.1 % (814 of 1333)	84.4 % (248 of 294)
Backbone	80.2 % (1325 of 1652)	54.2 % (303 of 559)	93.8 % (774 of 825)	92.5 % (248 of 268)
Sidechain	21.8 % (440 of 2016)	12.6 % (153 of 1215)	37.0 % (287 of 775)	0.0 % (0 of 26)
Aromatic	3.3 % (8 of 242)	5.0 % (6 of 121)	1.7 % (2 of 121)
Methyl	18.1 % (64 of 354)	18.1 % (32 of 177)	18.1 % (32 of 177)

1. Peroxisome Proliferator-Activated Receptor Gamma Ligand-Binding Domain

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	Peroxisome Proliferator-Activated Receptor Gamma Ligand-Binding Domain	[U-13C; U-15N; U-2H]	1.0 mM
2	potassium phosphate	natural abundance	20 mM
3	KCl	natural abundance	50 mM
4	NaN3	natural abundance	0.05 %
5	EDTA-d16	natural abundance	0.5 mM
6	b-mercaptoethanol-d6	natural abundance	8 mM
7	H20	natural abundance	95 %
8	D2O		5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.51 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.51 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.36 ppm, Outliers: 2 Detail

Release date

2008-01-10

Citation

Effect of heterodimer partner RXRalpha on PPARgamma activation function-2 helix in solution
Lu, J., Chen, M., Stanley, S.E., Li, E.
Biochem. Biophys. Res. Commun. (2008), 365, 42-46, PubMed 17980149 , DOI 10.1016/j.bbrc.2007.10.143 , Abstract

Related entities 1. Peroxisome Proliferator-Activated Receptor Gamma Ligand-Binding Domain, : 1 : 16 : 1 : 40 : 80 entities Detail

Interaction partners 1. Peroxisome Proliferator-Activated Receptor Gamma Ligand-Binding Domain, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC