BMRBj - BMREntryMaster

Found 1 Document (0.605 seconds)

BMRB: 15530

2VBS

Solution structure of Mj0056

Authors

Coles, M., Truffault, V., Djuranovic, S., Martin, J., Lupas, A.N.

Assembly

apo-Mj0056

Entity

1. apo-Mj0056 (polymer, Thiol state: not present), 136 monomers, 15685.31 Da Detail

MVKLMIIEGE VVSGLGEGRY FLSLPPYKEI FKKILGFEPY EGTLNLKLDR EFDINKFKYI ETEDFEFNGK RFFGVKVLPI KILIGNKKID GAIVVPKKTY HSSEIIEIIA PMKLREQFNL KDGDVIKILI KGDKDE

Formula weight

15685.31 Da

Source organism

Methanocaldococcus jannaschii

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.6 %, Completeness: 88.5 %, Completeness (bb): 88.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.5 % (1523 of 1720)	92.6 % (839 of 906)	83.6 % (567 of 678)	86.0 % (117 of 136)
Backbone	88.4 % (711 of 804)	89.9 % (250 of 278)	88.4 % (350 of 396)	85.4 % (111 of 130)
Sidechain	89.7 % (933 of 1040)	93.8 % (589 of 628)	83.3 % (338 of 406)	100.0 % (6 of 6)
Aromatic	54.9 % (79 of 144)	91.7 % (66 of 72)	18.1 % (13 of 72)
Methyl	99.4 % (165 of 166)	98.8 % (82 of 83)	100.0 % (83 of 83)

1. Riboflavin Kinase MJ0056

MVKLMIIEGE VVSGLGEGRY FLSLPPYKEI FKKILGFEPY EGTLNLKLDR EFDINKFKYI ETEDFEFNGK RFFGVKVLPI KILIGNKKID GAIVVPKKTY HSSEIIEIIA PMKLREQFNL KDGDVIKILI KGDKDE

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4, Details 0.65 mM MJ0056 UNLABELLED 30 mM PHOSPHATE BUFFER; 150 MM NACL 90% H2O,10% D2O

#	Name	Isotope labeling	Concentration
1	Riboflavin Kinase MJ0056	natural abundance	0.65 mM
2	sodium phosphate	natural abundance	30 mM
3	sodium chloride	natural abundance	150 mM
4	H2O		90 %
5	D2O		10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4, Details 0.65 mM MJ0056 UNLABELLED 30 mM PHOSPHATE BUFFER; 150 MM NACL 90% H2O,10% D2O

#	Name	Isotope labeling	Concentration
6	Riboflavin Kinase MJ0056	[U-100% 15N]	0.65 mM
7	sodium phosphate	natural abundance	30 mM
8	sodium chloride	natural abundance	150 mM
9	H2O		90 %
10	D2O		10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 7.4, Details 0.65 mM MJ0056 UNLABELLED 30 mM PHOSPHATE BUFFER; 150 MM NACL 90% H2O,10% D2O

#	Name	Isotope labeling	Concentration
11	Riboflavin Kinase MJ0056	[U-100% 13C; U-100% 15N]	0.65 mM
12	sodium phosphate	natural abundance	30 mM
13	sodium chloride	natural abundance	150 mM
14	H2O		90 %
15	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.15 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.15 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.57 ppm, Outliers: 2 Detail

Release date

2008-06-26

Citation

A CTP-dependent archaeal riboflavin kinase forms a bridge in the evolution of cradle-loop barrels
Ammelburg, M., Hartmann, M.D., Djuranovic, S., Alva, V., Koretke, K.K., Martin, J., Sauer, G., Truffault, V., Zeth, K., Lupas, A.N., Coles, M.
Structure (2007), 15, 1577-1590, PubMed 18073108 , DOI 10.1016/j.str.2007.09.027 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: Q60365 released on 1997-11-01
Title RIFK_METJA Entity Riboflavin kinase

Related entities 1. apo-Mj0056, : 1 : 4 : 1 : 2 : 54 entities Detail

Experiments performed 11 experiments Detail

1 2D NOESY