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Chemical Shift Assignment of the Transmembrane Helices of DsbB by 3D Magic Angle Spinning NMR
Authors
Li, Y., Berthold, D.A., Frericks-Schmidt, H.L., Gennis, R.B., Rienstra, C.M.
Assembly
DsbB-ubiquinone
Entity
1. DsbB (polymer, Thiol state: free and disulfide bound), 186 monomers, 21300.04 Da Detail

MLRFLNQASQ GRGAWLLMAF TALALELTAL WFQHVMLLKP SVLCIYERVA LFGVLGAALI GAIAPKTPLR YVAMVIWLYS AFRGVQLTYE HTMLQLYPSP FATCDFMVRF PEWLPLDKWV PQVFVASGDC AERQWDFLGL EMPQWLLGIF IAYLIVAVLV VISQPFKAKK RDLFGRGSRS HHHHHH


2. UQ8 (non-polymer), 727.109 Da
Total weight
22027.148 Da
Max. entity weight
21300.04 Da
Entity Connection
disulfide 1 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1disulfidesing1:CYS104:SG1:CYS130:SG

Source organism
Escherichia coli
Exptl. method
solid-state NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 35.5 %, Completeness: 24.5 %, Completeness (bb): 32.0 % Detail

Polymer type: polypeptide(L)

Total13C15N
All24.5 % (275 of 1121)23.3 % (214 of 918)30.0 % (61 of 203)
Backbone32.0 % (231 of 723)31.1 % (170 of 547)34.7 % (61 of 176)
Sidechain16.2 % (93 of 573)17.0 % (93 of 546) 0.0 % (0 of 27)
Aromatic 0.7 % (1 of 147) 0.7 % (1 of 140) 0.0 % (0 of 7)
Methyl28.3 % (36 of 127)28.3 % (36 of 127)

1. DsbB

MLRFLNQASQ GRGAWLLMAF TALALELTAL WFQHVMLLKP SVLCIYERVA LFGVLGAALI GAIAPKTPLR YVAMVIWLYS AFRGVQLTYE HTMLQLYPSP FATCDFMVRF PEWLPLDKWV PQVFVASGDC AERQWDFLGL EMPQWLLGIF IAYLIVAVLV VISQPFKAKK RDLFGRGSRS HHHHHH

Sample

Pressure 1 atm, pH 7.5


#NameIsotope labelingTypeConcentration
1DsbB[U-100% 13C; U-100% 15N]20 mg

Release date
2008-06-26
Citation
Chemical shift assignment of the transmembrane helices of DsbB, a 20-kDa integral membrane enzyme, by 3D magic-angle spinning NMR spectroscopy
Li, Y., Berthold, D.A., Gennis, R.B., Rienstra, C.M.
Protein Sci. (2008), 17, 199-204, PubMed 18227427 , DOI 10.1110/ps.073225008 ,
Related entities 1. DsbB, : 1 : 5 : 54 entities Detail
Interaction partners 1. DsbB, : 1 interactors Detail