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Found 1 Document (2.127 seconds)

BMRB: 15563

2H8H

Backbone 1H, 13C and 15N chemical shift assignment of N-terminal end (1-85) of human SRC

Authors

Perez, Y., Bernad, P., Gair, M., Pons, M.

Assembly

USRC

Entity

1. USRC (polymer, Thiol state: not present), 95 monomers, 9626.415 Da Detail

MASNKSKPKD ASQRRRSLEP AENVHGAGGG AFPASQTPSK PASADGHRGP SAAFAPAAAE PKLFGGFNSS DTVTSPQRAG PLAGGSAWSH PQFEK

Formula weight

9626.415 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 47.1 %, Completeness (bb): 80.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	47.1 % (473 of 1005)	23.3 % (123 of 527)	69.4 % (268 of 386)	89.1 % (82 of 92)
Backbone	80.7 % (442 of 548)	50.0 % (95 of 190)	97.1 % (266 of 274)	96.4 % (81 of 84)
Sidechain	20.7 % (112 of 541)	8.3 % (28 of 337)	42.3 % (83 of 196)	12.5 % (1 of 8)
Aromatic	0.0 % (0 of 74)	0.0 % (0 of 37)	0.0 % (0 of 36)	0.0 % (0 of 1)
Methyl	36.7 % (22 of 60)	16.7 % (5 of 30)	56.7 % (17 of 30)

1. USRC

MASNKSKPKD ASQRRRSLEP AENVHGAGGG AFPASQTPSK PASADGHRGP SAAFAPAAAE PKLFGGFNSS DTVTSPQRAG PLAGGSAWSH PQFEK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 4.5, Details 0.6 mM USRC in 50 mM acetate pH=4.5

#	Name	Isotope labeling	Concentration
1	USRC	[U-100% 13C; U-100% 15N]	0.6 mM
2	PMSF	natural abundance	0.2 mM
3	sodium acetate	natural abundance	50 mM
4	D2O	[U-99% 2H]	10 %
5	H2O	natural abundance	90 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.74 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 2.74 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 3.07 ppm, Outliers: 1 Detail

Release date

2009-06-28

Citation

Structural characterization of the natively unfolded N-terminal domain of human c-Src kinase: insights into the role of phosphorylation of the unique domain
Perez, Y., Gairi, M., Pons, M., Bernado, P.
J. Mol. Biol. (2009), 391, 136-148, PubMed 19520085 , DOI 10.1016/j.jmb.2009.06.018 , Abstract

Related entities 1. USRC, : 1 : 2 : 25 entities Detail

Interaction partners 1. USRC, : 210 interactors Detail

More details for 210 interactors identified by 92 citations

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr15563_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr15563_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	437		98.9 (chain: 1, length: 95)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 95, Sequence coverage: 98.9%
- Total number of assignments
  - ¹³C chemical shifts: 266
  - ¹H chemical shifts: 80
  - ¹⁵N chemical shifts: 91
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
8	PRO	N	137.491
20	PRO	N	137.484
33	PRO	N	137.342
38	PRO	N	139.23
41	PRO	N	137.407
50	PRO	N	134.173
56	PRO	N	135.327
61	PRO	N	137.515
76	PRO	N	137.786
81	PRO	N	133.859
91	PRO	N	137.024

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	386	266	68.9
¹H chemical shifts	527	80	15.2
¹⁵N chemical shifts	97	80	82.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	190	185	97.4
¹H chemical shifts	190	80	42.1
¹⁵N chemical shifts	84	80	95.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	196	81	41.3
¹H chemical shifts	337	0	0.0
¹⁵N chemical shifts	13	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	31	17	54.8
¹H chemical shifts	31	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	36	0	0.0
¹H chemical shifts	37	0	0.0
¹⁵N chemical shifts	1	0	0.0

Keywords SRC, unfolded

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Found 1 Document (2.127 seconds)

BMRB: 15563

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. USRC, : 1 : 2 : 25 entities Detail

Interaction partners 1. USRC, : 210 interactors Detail

Experiments performed 4 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail