BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	85.1 % (940 of 1104)	85.8 % (497 of 579)	81.8 % (350 of 428)	95.9 % (93 of 97)
Backbone	97.6 % (562 of 576)	99.0 % (199 of 201)	96.8 % (274 of 283)	96.7 % (89 of 92)
Sidechain	75.6 % (465 of 615)	78.8 % (298 of 378)	70.3 % (163 of 232)	80.0 % (4 of 5)
Aromatic	39.4 % (26 of 66)	78.8 % (26 of 33)	0.0 % (0 of 33)
Methyl	87.8 % (79 of 90)	95.6 % (43 of 45)	80.0 % (36 of 45)

1. PDZ2

GIDPFTMLRP RLCTMKKGPS GYGFNLHSDK SKPGQFIRSV DPDSPAEASG LRAQDRIVEV NGVCMEGKQH GDVVSAIRAG GDETKLLVVD RETDEFFK

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 288.1 K, pH 7.5, Details 1 mM, NHERF PDZ2

#	Name	Isotope labeling	Concentration
1	PDZ2	natural abundance	1 mM
2	NaCl	natural abundance	150 mM
3	HEPES	natural abundance	20 mM
4	DTT	natural abundance	0.5 mM
5	D2O	[U-100% 2H]	5 %
6	H2O		95 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 288.1 K, pH 7.5, Details 1mM, 1H, 15N NHERF PDZ2

#	Name	Isotope labeling	Concentration
7	PDZ2	[U-100% 15N]	1 mM
8	NaCl	natural abundance	150 mM
9	HEPES	natural abundance	20 mM
10	DTT	natural abundance	0.5 mM
11	D2O	[U-100% 2H]	5 %
12	H2O		95 %

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 288.1 K, pH 7.5, Details 1 mM, 1H, 13C, 15N NHERF PDZ2

#	Name	Isotope labeling	Concentration
13	PDZ2	[U-100% 13C; U-100% 15N]	1 mM
14	NaCl	natural abundance	150 mM
15	HEPES	natural abundance	20 mM
16	DTT	natural abundance	0.5 mM
17	D2O	[U-100% 2H]	5 %
18	H2O		95 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.64 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 12 models in PDB: 2JXO, Strand ID: A Detail

Release date

2009-05-27

Citation

Autoinhibitory interactions between the PDZ2 and C-terminal domains in the scaffolding protein NHERF1
Cheng, H., Li, J., Ruzaliya, F., Dai, Z., Bu, Z., Roder, H.
Structure (2009), 17, 660-669, PubMed 19446522 , DOI 10.1016/j.str.2009.03.009 , Abstract

Related entities 1. PDZ domain, : 1 : 3 : 4 : 330 entities Detail

Interaction partners 1. PDZ domain, : 35 interactors Detail

More details for 35 interactors identified by 22 citations

Experiments performed 12 experiments Detail

1 2D 1H-1H TOCSY

Instrument

Bruker DMX - 600 MHz

Sample

State anisotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 288.1 K, pH 7.5, Details 1 mM, NHERF PDZ2

#	Name	Isotope labeling	Concentration
1	PDZ2	natural abundance	1 mM
2	NaCl	natural abundance	150 mM
3	HEPES	natural abundance	20 mM
4	DTT	natural abundance	0.5 mM
5	D2O	[U-100% 2H]	5 %
6	H2O		95 %

2 2D 1H-1H NOESY

Instrument

Bruker DMX - 600 MHz

Sample

State anisotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 288.1 K, pH 7.5, Details 1 mM, NHERF PDZ2

#	Name	Isotope labeling	Concentration
1	PDZ2	natural abundance	1 mM
2	NaCl	natural abundance	150 mM
3	HEPES	natural abundance	20 mM
4	DTT	natural abundance	0.5 mM
5	D2O	[U-100% 2H]	5 %
6	H2O		95 %

3 2D 1H-15N HSQC

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 288.1 K, pH 7.5, Details 1mM, 1H, 15N NHERF PDZ2

#	Name	Isotope labeling	Concentration
7	PDZ2	[U-100% 15N]	1 mM
8	NaCl	natural abundance	150 mM
9	HEPES	natural abundance	20 mM
10	DTT	natural abundance	0.5 mM
11	D2O	[U-100% 2H]	5 %
12	H2O		95 %

4 3D 1H-15N NOESY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 288.1 K, pH 7.5, Details 1mM, 1H, 15N NHERF PDZ2

#	Name	Isotope labeling	Concentration
7	PDZ2	[U-100% 15N]	1 mM
8	NaCl	natural abundance	150 mM
9	HEPES	natural abundance	20 mM
10	DTT	natural abundance	0.5 mM
11	D2O	[U-100% 2H]	5 %
12	H2O		95 %

5 3D 1H-15N TOCSY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 288.1 K, pH 7.5, Details 1mM, 1H, 15N NHERF PDZ2

#	Name	Isotope labeling	Concentration
7	PDZ2	[U-100% 15N]	1 mM
8	NaCl	natural abundance	150 mM
9	HEPES	natural abundance	20 mM
10	DTT	natural abundance	0.5 mM
11	D2O	[U-100% 2H]	5 %
12	H2O		95 %

6 3D HNHA

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 288.1 K, pH 7.5, Details 1mM, 1H, 15N NHERF PDZ2

#	Name	Isotope labeling	Concentration
7	PDZ2	[U-100% 15N]	1 mM
8	NaCl	natural abundance	150 mM
9	HEPES	natural abundance	20 mM
10	DTT	natural abundance	0.5 mM
11	D2O	[U-100% 2H]	5 %
12	H2O		95 %

7 3D HNCO