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Backbone H, N and CA Chemical Shift Assignments for ApoMb(1-77) in Complex with DnaK Binding Domain
Authors
Kurt, N., Cavagnero, S.
Assembly
Sperm Whale apoMb(1-77) complex with DnaK-beta
Entity
1. Apomyoglobin (1-77) (polymer, Thiol state: not available), 77 monomers, 8687.970 Da Detail

VLSEGEWQLV LHVWAKVEAD VAGHGQDILI RLFKSHPETL EKFDRFKHLK TEAEMKASED LKKHGVTVLT ALGAILK


2. DnaK-beta (polymer), na Da
Formula weight
8687.97 Da
Source organism
Physeter catodon , Escherichia coli
Exptl. method
SOLUTION NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 62.3 %, Completeness: 16.3 %, Completeness (bb): 31.6 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All16.3 % (151 of 924)10.9 % (52 of 476)14.2 % (52 of 366)57.3 % (47 of 82)
Backbone31.6 % (144 of 455)29.4 % (45 of 153)23.0 % (52 of 226)61.8 % (47 of 76)
Sidechain 1.7 % (9 of 541) 2.2 % (7 of 323) 0.9 % (2 of 212) 0.0 % (0 of 6)
Aromatic 0.0 % (0 of 74) 0.0 % (0 of 37) 0.0 % (0 of 35) 0.0 % (0 of 2)
Methyl 1.9 % (2 of 106) 1.9 % (1 of 53) 1.9 % (1 of 53)

1. Apomyoglobin (1-77)

VLSEGEWQLV LHVWAKVEAD VAGHGQDILI RLFKSHPETL EKFDRFKHLK TEAEMKASED LKKHGVTVLT ALGAILK

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 277 K, pH 5.8 (±0.1), Details ApoMb(1-77) was first dissolved in HCl at pH 2.5, then diluted into buffer containing DnaKbeta


#NameIsotope labelingTypeConcentration
1Apomyoglobin (1-77)[U-99% 13C; U-99% 15N]100 uM
2DnaK-beta[U-2H]100 uM
3sodium acetatenatural abundance10 mM
4D2O[U-100% 2H]5 %
5H2O95 %

Release date
2008-06-29
Citation
Nonnative helical motif in a chaperone-bound protein fragment
Kurt, N., Cavagnero, S.
Biophys. J. (2008), 94, L48-L50, PubMed 18192369 , DOI 10.1529/biophysj.107.127647 ,
Related entities 1. Apomyoglobin (1-77), : 1 : 100 : 99 entities Detail
Interaction partners 1. Apomyoglobin (1-77), : 2 interactors Detail
Experiments performed 2 experiments Detail