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BMRB: 15654

8EYR

NMR Chemical Shift Assignments of Insulin-like Growth Factor-I (IGF-I)

Authors

Kuang, Z., Yao, S., McNeil, K.A., Forbes, B.E., Wallace, J.C., Norton, R.S.

Assembly

Insulin-like growth factor-I (IGF-I)

Entity

1. Insulin-like growth factor-I (IGF-I) (polymer, Thiol state: all disulfide bound), 70 monomers, 7654.677 Da Detail

GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA

Formula weight

7654.677 Da

Entity Connection

disulfide 3 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS6:SG	1:CYS48:SG
2	disulfide	sing	1:CYS18:SG	1:CYS61:SG
3	disulfide	sing	1:CYS47:SG	1:CYS52:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.6 %, Completeness: 66.5 %, Completeness (bb): 96.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	66.5 % (518 of 779)	62.9 % (256 of 407)	65.5 % (199 of 304)	92.6 % (63 of 68)
Backbone	96.3 % (395 of 410)	96.5 % (137 of 142)	96.1 % (195 of 203)	96.9 % (63 of 65)
Sidechain	42.1 % (182 of 432)	44.9 % (119 of 265)	38.4 % (63 of 164)	0.0 % (0 of 3)
Aromatic	0.0 % (0 of 64)	0.0 % (0 of 32)	0.0 % (0 of 32)
Methyl	19.0 % (11 of 58)	20.7 % (6 of 29)	17.2 % (5 of 29)

1. Insulin-like growth factor-I (IGF-I)

GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 310 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	Insulin-like growth factor-I (IGF-I)	[U-98% 13C; U-98% 15N]	0.7 mM
2	H2O	natural abundance	95 %
3	D2O	[U-100% 2H]	5 %
4	sodium acetate	natural abundance	10 mM
5	sodium azide	natural abundance	0.02% w/v

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.36 ppm, Outliers: 1 Detail

Release date

2009-02-08

Citation

Insulin-like growth factor-I (IGF-I): solution properties and NMR chemical shift assignments near physiological pH
Kuang, Z., Yao, S., McNeil, K.A., Forbes, B.E., Wallace, J.C., Norton, R.S.
Growth. Horm. IGF Res. (2009), 19, 226-231, PubMed 19056307 , DOI 10.1016/j.ghir.2008.10.003 , Abstract

Related entities 1. Insulin-like growth factor-I (IGF-I), : 1 : 30 : 3 : 6 : 155 entities Detail

Interaction partners 1. Insulin-like growth factor-I (IGF-I), : 9 interactors Detail

More details for 9 interactors identified by 4 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC