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BMRB: 15682

2K2T

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Epidermal Growth Factor-like 2 domain from Microneme Protein 6 (Toxoplasma gondii)

Authors

Sawmynaden, K., Saouros, S., Marchant, J., Simpson, P., Matthews, S.

Assembly

Epidermal Growth Factor-like domain 2

Entity

1. Epidermal Growth Factor-like domain 2 (polymer, Thiol state: all disulfide bound), 61 monomers, 6362.020 Da Detail

MANFVQLSET PAACSSNPCG PEAAGTCKET NSGYICRCNQ GYRISLDGTG NVTCIVRQES G

Formula weight

6362.02 Da

Entity Connection

disulfide 3 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS14:SG	1:CYS27:SG
2	disulfide	sing	1:CYS19:SG	1:CYS36:SG
3	disulfide	sing	1:CYS38:SG	1:CYS54:SG

Source organism

Toxoplasma gondii

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.7 %, Completeness: 87.5 %, Completeness (bb): 91.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.5 % (552 of 631)	86.5 % (282 of 326)	89.5 % (214 of 239)	84.8 % (56 of 66)
Backbone	91.7 % (330 of 360)	89.7 % (113 of 126)	93.2 % (164 of 176)	91.4 % (53 of 58)
Sidechain	84.3 % (274 of 325)	85.0 % (170 of 200)	86.3 % (101 of 117)	37.5 % (3 of 8)
Aromatic	69.2 % (18 of 26)	76.9 % (10 of 13)	61.5 % (8 of 13)
Methyl	82.7 % (43 of 52)	84.6 % (22 of 26)	80.8 % (21 of 26)

1. Epidermal Growth Factor-like domain 2

MANFVQLSET PAACSSNPCG PEAAGTCKET NSGYICRCNQ GYRISLDGTG NVTCIVRQES G

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Temperature 303 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	Epidermal Growth Factor-like domain 2	[U-98% 13C; U-98% 15N]	0.5 mM
2	potassium phosphate	natural abundance	25 mM
3	sodium chloride	natural abundance	50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.725 ppm	internal	direct	1.0
¹⁵N	water	protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 6 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.725 ppm	internal	direct	1.0
¹⁵N	water	protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 6 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.725 ppm	internal	direct	1.0
¹⁵N	water	protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.725 ppm	internal	direct	1.0
¹⁵N	water	protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.21 ppm, Outliers: 2 Detail

Release date

2008-10-02

Citation

Complete NMR assignments for the second EGF domain of MIC6 from Toxoplasma gondii and re-assignment in complex with the galectin-like domain of MIC1
Sawmynaden, K., Saouros, S., Marchant, J., Simpson, P., Matthews, S.
Biomol. NMR Assign. (2008), 2, 187-189, PubMed 19636901 , DOI 10.1007/s12104-008-9117-5 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 15685 released on 2008-10-02
Title Backbone and side chain 1H, 13C, and 15N Chemical Shift Assignments for the complex of Epidermal Growth Factor-like 2 domain from Microneme Protein 6 (Toxoplasma gondii) with the Galectin-like domain of Microneme protein 1

Related entities 1. Epidermal Growth Factor-like domain 2, : 1 : 2 : 3 entities Detail

Interaction partners 1. Epidermal Growth Factor-like domain 2, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC