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Automated NMR Structure of the UBB by FAPSY
Authors
Lee, W., Jung, J., Lee, W.
Assembly
UBB
Entity
1. UBB (polymer, Thiol state: unknown), 103 monomers, 11521.82 Da Detail

GSLVPRGSMQ IFVKTLTGKT ITLEVEPSDT IENVKAKIQD KEGIPPDQQR LIFAGKQLED GRTLSEYNIQ KESTLHLVLR LRGYADLRED PDRQDHHPGS GAQ


Formula weight
11521.82 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 96.1 %, Completeness: 75.9 %, Completeness (bb): 81.4 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All75.9 % (915 of 1206)81.0 % (512 of 632)67.6 % (311 of 460)80.7 % (92 of 114)
Backbone81.4 % (486 of 597)96.0 % (192 of 200)67.3 % (202 of 300)94.8 % (92 of 97)
Sidechain73.8 % (519 of 703)74.1 % (320 of 432)78.3 % (199 of 254) 0.0 % (0 of 17)
Aromatic20.8 % (10 of 48)25.0 % (6 of 24)16.7 % (4 of 24)
Methyl90.4 % (103 of 114)91.2 % (52 of 57)89.5 % (51 of 57)

1. UBB

GSLVPRGSMQ IFVKTLTGKT ITLEVEPSDT IENVKAKIQD KEGIPPDQQR LIFAGKQLED GRTLSEYNIQ KESTLHLVLR LRGYADLRED PDRQDHHPGS GAQ

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0


#NameIsotope labelingTypeConcentration
1UBB[U-98% 13C; U-98% 15N]1 mM

LACS Plot; CA
Referencing offset: -0.04 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.04 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.02 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2K25, Strand ID: A Detail


Release date
2009-05-11
Citation
The Automated Suite for Protein Structure by NMR Spectroscopy
Lee, W., Jung, J., Lee, W.
Related entities 1. UBB, : 2 entities Detail
Experiments performed 8 experiments Detail
Keywords AUTOMATION, FAPSY, MOAD, MOLYBDOPTERIN