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BMRB: 15703

8G94

Soluble form of human earliest activation antigen of leukocytes CD69

Authors

Kubickova, M., Kaderavek, P., Chmelik, J., Zidek, L., Sklenar, V.

Assembly

CD69

Entity

1. CD69 (polymer, Thiol state: not reported), 130 monomers, 15124.79 Da Detail

GQYTFSMPSD SHVSSCSEDW VGYQRKCYFI STVKRSWTSA QNACSEHGAT LAVIDSEKDM NFLKRYAGRE EHWVGLKKEP GHPWKWSNGK EFNNWFNVTG SDKCVFLKNT EVSSMECEKN LYWICNKPYK

Formula weight

15124.79 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, heteronucl_NOEs, heteronucl_T1_relaxation, heteronucl_T2_relaxation

Chem. Shift Complete

Sequence coverage: 86.9 %, Completeness: 30.3 %, Completeness (bb): 58.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	30.3 % (472 of 1556)	16.6 % (135 of 812)	38.7 % (232 of 599)	72.4 % (105 of 145)
Backbone	58.5 % (452 of 772)	45.5 % (120 of 264)	59.4 % (227 of 382)	83.3 % (105 of 126)
Sidechain	13.5 % (122 of 906)	2.7 % (15 of 548)	31.6 % (107 of 339)	0.0 % (0 of 19)
Aromatic	0.0 % (0 of 208)	0.0 % (0 of 104)	0.0 % (0 of 97)	0.0 % (0 of 7)
Methyl	17.4 % (15 of 86)	14.0 % (6 of 43)	20.9 % (9 of 43)

1. CD69

GQYTFSMPSD SHVSSCSEDW VGYQRKCYFI STVKRSWTSA QNACSEHGAT LAVIDSEKDM NFLKRYAGRE EHWVGLKKEP GHPWKWSNGK EFNNWFNVTG SDKCVFLKNT EVSSMECEKN LYWICNKPYK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 5.8

#	Name	Isotope labeling	Concentration
1	CD69	[U-13C; U-15N]	0.9 mM
2	sodium azide	natural abundance	1 mM
3	D2O	[U-99% 2H]	10 %
4	sodium chloride	natural abundance	50 mM
5	MES	natural abundance	10 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K, pH 5.8

#	Name	Isotope labeling	Concentration
6	CD69	[U-15N]	0.65 mM
7	sodium azide	natural abundance	1 mM
8	D2O	[U-99% 2H]	10 %
9	sodium chloride	natural abundance	50 mM
10	MES	natural abundance	10 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

Heteronucl. T₁

83 T₁ values in 1 lists
Coherence Sz, Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 300 K, pH 5.8 Detail

Heteronucl. T₂

84 T₂ values in 1 lists
Coherence S(+,-), Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 300 K, pH 5.8 Detail

Heteronucl. NOE

83 NOE values in 1 lists
Value type peak height, Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 300 K, pH 5.8 Detail

Heteronucl. T₁/T₂

83 T₁/T₂ values in 1 lists
Field strength (¹H) 600 MHz, Pressure 1 atm, Temperature 300 K, pH 5.8 Detail

Release date

2008-11-09

Citation

Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemical stability display prolonged circulation and remain intact in the blood of mice
Vanek, O., Nalezkova, M., Kavan, D., Borovickova, I., Pompach, P., Novak, P., Kumar, V., Vannucci, L., Hudecek, J., Hofbauerova, K., Kopecky, V., Brynda, J., Kolenko, P., Dohnalek, J., Kaderavek, P., Chmelik, J., Gorcik, L., Zidek, L., Sklenar, V., Bezouska, K.
FEBS J. (2008), 275, 5589-5606, PubMed 18959746 , DOI 10.1111/j.1742-4658.2008.06683.x , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: Q07108 released on 1994-10-01
Title CD69_HUMAN Entity Early activation antigen CD69

Related entities 1. CD69, : 1 : 4 : 3 : 257 entities Detail

Interaction partners 1. CD69, : 3 interactors Detail

More details for 3 interactors identified by 3 citations

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC