BMRBj - BMREntryMaster

Found 1 Document (0.687 seconds)

BMRB: 15715

2L1U

NMR Assignments of Methionine Sulfoxide Reductase B2 from Homo sapiens

Authors

Breivik, A.S., Aachmann, F.L., Dikiy, A.

Assembly

MsrB2dS

Entity

1. Methionine Sulfoxide Reductase B2 (polymer, Thiol state: all free), 143 monomers, 16002.97 Da Detail

MADWQKKLTP EQFYVTREKG TEAPFSGMYL NNKETGMYHC VCCDSPLFSS EKKYCSGTGW PSFSEAYGSK GSDESHTGIL RRLDTSLGCP RMEVVCKQCE AHLGHVFPDG PKPTGQRFCI NSVALKFKPS KPAAALEHHH HHH

2. ZN (non-polymer), 65.409 Da

Total weight

16068.379 Da

Max. entity weight

16002.97 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.5 %, Completeness: 84.1 %, Completeness (bb): 95.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.1 % (1382 of 1644)	84.1 % (722 of 859)	82.0 % (527 of 643)	93.7 % (133 of 142)
Backbone	95.2 % (798 of 838)	95.8 % (276 of 288)	95.0 % (396 of 417)	94.7 % (126 of 133)
Sidechain	75.7 % (709 of 937)	78.1 % (446 of 571)	71.7 % (256 of 357)	77.8 % (7 of 9)
Aromatic	12.1 % (21 of 174)	19.5 % (17 of 87)	4.7 % (4 of 85)	0.0 % (0 of 2)
Methyl	99.0 % (99 of 100)	100.0 % (50 of 50)	98.0 % (49 of 50)

1. Methionine Sulfoxide Reductase B2

MADWQKKLTP EQFYVTREKG TEAPFSGMYL NNKETGMYHC VCCDSPLFSS EKKYCSGTGW PSFSEAYGSK GSDESHTGIL RRLDTSLGCP RMEVVCKQCE AHLGHVFPDG PKPTGQRFCI NSVALKFKPS KPAAALEHHH HHH

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 (±0.5) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Methionine Sulfoxide Reductase B2	[U-98% 13C; U-98% 15N]	1.1 (±0.1) mM
2	Zinc ion	natural abundance	1.1 (±0.1) mM
3	DTT	natural abundance	5 (±0.1) mM
4	sodium phosphate	natural abundance	20 (±0.5) mM
5	sodium chloride	natural abundance	25 (±0.5) mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.5) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
6	Methionine Sulfoxide Reductase B2	[U-98% 13C; U-98% 15N]	1.1 (±0.1) mM
7	Zinc ion	natural abundance	1.1 (±0.1) mM
8	DTT	natural abundance	5 (±0.1) mM
9	sodium phosphate	natural abundance	20 (±0.5) mM
10	sodium chloride	natural abundance	25 (±0.5) mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.75 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.14 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.75 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.14 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.75 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.75 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.23 ppm, Outliers: 2 Detail

Release date

2008-10-14

Citation

1H, 15N and 13C NMR assignments of mouse methionine sulfoxide reductase B2
Breivik, A.S., Aachmann, F.L., Sal, L.S., Kim, H., Del Conte, R., Gladyshev, V.N., Dikiy, A.
Biomol. NMR Assign. (2008), 2, 199-201, PubMed 19636904 , DOI 10.1007/s12104-008-9120-x , Abstract

Related entities 1. Methionine Sulfoxide Reductase B2, : 1 : 2 : 157 entities Detail

Interaction partners 1. Methionine Sulfoxide Reductase B2, : 1 interactors Detail

More details for 1 interactors identified by 1 citations