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BMRB: 15727

2VOD

Full 1H, 13C, and 15N Chemical Shift Assignments for La NTD complexed with 5'UUUU RNA

Authors

Conte, M.R., Sanfelice, D., Kelly, G., Curry, S.

Assembly

La NTD complexed with 5'UUUU

Entity

1. La protein (polymer, Thiol state: all free), 197 monomers, 22983.04 Da Detail

MAENGDNEKM AALEAKICHQ IEYYFGDFNL PRDKFLKEQI KLDEGWVPLE IMIKFNRLNR LTTDFNVIVE ALSKSKAELM EISEDKTKIR RSPSKPLPEV TDEYKNDVKN RSVYIKGFPT DATLDDIKEW LEDKGQVLNI QMRRTLHKAF KGSIFVVFDS IESAKKFVET PGQKYKETDL LILFKDDYFA KKNEVDS

2. UUUU RNA (polymer, Thiol state: not present), 4 monomers, 1242.678 Da Detail

UUUU

Total weight

24225.717 Da

Max. entity weight

22983.04 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.0 %, Completeness: 78.1 %, Completeness (bb): 90.6 % Detail

Polymer type: polypeptide(L) polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	78.1 % (1952 of 2499)	73.7 % (969 of 1315)	82.1 % (799 of 973)	87.2 % (184 of 211)
Backbone	90.6 % (1098 of 1212)	87.1 % (364 of 418)	91.2 % (551 of 604)	96.3 % (183 of 190)
Sidechain	70.1 % (1035 of 1477)	67.4 % (605 of 897)	76.7 % (429 of 559)	4.8 % (1 of 21)
Aromatic	36.2 % (81 of 224)	39.3 % (44 of 112)	34.9 % (37 of 106)	0.0 % (0 of 6)
Methyl	95.1 % (196 of 206)	95.1 % (98 of 103)	95.1 % (98 of 103)

1. La protein

2. UUUU RNA

UUUU

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7

#	Name	Isotope labeling	Concentration
1	La protein	[U-100% 13C; U-100% 15N]	0.3-0.4 mM
2	5'UUUU RNA		0.3-0.4 mM
3	Tris-HCl		20 mM
4	KCl		100 mM
5	DTT		1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 2 Detail

Release date

2008-11-10

Citation

NMR assignment of the N-terminal region of human La free and in complex with RNA
Sanfelice, D., Kelly, G., Curry, S., Conte, M.R.
Biomol. NMR Assign. (2008), 2, 107-109, PubMed 19636881 , DOI 10.1007/s12104-008-9097-5 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 15726 released on 2008-11-10
Title Backbone 1H, 13C, and 15N Chemical Shift Assignments for La NTD

Related entities 1. La protein, : 1 : 7 : 37 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC