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BMRB: 15764

3US9

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Ca2+-Binding Domain 1 of the Na+/Ca2+ Exchanger in the Absence of Ca2+

Authors

Johnson, E., Bruschweiler-Li, L., Showalter, S.A., Vuister, G.W., Zhang, F., Bruschweiler, R.

Assembly

CBD1

Entity

1. CBD1 (polymer, Thiol state: all free), 147 monomers, 16193.52 Da Detail

MSHHHHHHVS KIFFEQGTYQ CLENCGTVAL TIIRRGGDLT NTVFVDFRTE DGTANAGSDY EFTEGTVVFK PGETQKEIRV GIIDDDIFEE DENFLVHLSN VKVSSEASED GILEANHVSA LACLGSPSTA TVTIFDDDHA GIFTFEE

Formula weight

16193.52 Da

Source organism

Canis lupus familiaris

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.2 %, Completeness: 40.8 %, Completeness (bb): 71.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	40.8 % (662 of 1621)	19.7 % (162 of 821)	57.6 % (372 of 646)	83.1 % (128 of 154)
Backbone	71.3 % (626 of 878)	45.7 % (139 of 304)	83.7 % (359 of 429)	88.3 % (128 of 145)
Sidechain	15.7 % (138 of 878)	4.6 % (24 of 517)	32.4 % (114 of 352)	0.0 % (0 of 9)
Aromatic	0.0 % (0 of 162)	0.0 % (0 of 81)	0.0 % (0 of 81)
Methyl	15.1 % (25 of 166)	10.8 % (9 of 83)	19.3 % (16 of 83)

1. CBD1

MSHHHHHHVS KIFFEQGTYQ CLENCGTVAL TIIRRGGDLT NTVFVDFRTE DGTANAGSDY EFTEGTVVFK PGETQKEIRV GIIDDDIFEE DENFLVHLSN VKVSSEASED GILEANHVSA LACLGSPSTA TVTIFDDDHA GIFTFEE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 306 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	CBD1	[U-100% 13C; U-100% 15N]	0.5 mM
2	HEPES	natural abundance	20 mM
3	beta-mercaptoethanol	natural abundance	20 mM
4	sodium azide	natural abundance	0.02 %
5	EDTA	natural abundance	15 mM
6	sodium chloride	natural abundance	100 mM
7	H2O	natural abundance	90 %
8	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.697 ppm	internal	indirect	0.2514495
¹H	water	protons	4.697 ppm	internal	direct	1.0
¹⁵N	water	protons	4.697 ppm	internal	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.697 ppm	internal	indirect	0.2514495
¹H	water	protons	4.697 ppm	internal	direct	1.0
¹⁵N	water	protons	4.697 ppm	internal	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.697 ppm	internal	indirect	0.2514495
¹H	water	protons	4.697 ppm	internal	direct	1.0
¹⁵N	water	protons	4.697 ppm	internal	indirect	0.1013291

Referencing offset: 0.66 ppm, Outliers: 1 Detail

Release date

2008-06-29

Citation

Structure and dynamics of Ca2+-binding domain 1 of the Na+/Ca2+ exchanger in the presence and in the absence of Ca2+
Johnson, E., Bruschweiler-Li, L., Showalter, S.A., Vuister, G.W., Zhang, F., Bruschweiler, R.
J. Mol. Biol. (2008), 377, 945-955, PubMed 18280495 , DOI 10.1016/j.jmb.2008.01.046 , Abstract

Related entities 1. CBD1, : 1 : 2 : 104 entities Detail

Interaction partners 1. CBD1, : 1 interactors Detail

More details for 1 interactors identified by 1 citations