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Deletion of the omega-loop in the Active Site of Staphylococcal Nuclease 2. Effects on Protein Structure and Dynamics
Authors
Baldisseri, D.M., Torchia, D.A., Poole, L.B., Gerlt, J.A.
Assembly
micrococcal nuclease
Source organism
Staphylococcus aureus
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 86.5 %, Completeness: 26.7 %, Completeness (bb): 64.3 % Detail

Polymer type: polypeptide(L)

Total1H15N
All26.7 % (287 of 1073)16.6 % (152 of 916)86.0 % (135 of 157)
Backbone64.3 % (270 of 420)47.9 % (135 of 282)97.8 % (135 of 138)
Sidechain 2.6 % (17 of 653) 2.7 % (17 of 634) 0.0 % (0 of 19)
Aromatic 0.0 % (0 of 58) 0.0 % (0 of 57) 0.0 % (0 of 1)
Methyl 2.8 % (2 of 71) 2.8 % (2 of 71)

1. micrococcal nuclease

XXXXXXXXXX XXKLHKEPAT LIKAIDGDTV KLMYKGQPMT FRLLLVDTPE TKHPKKGVEK YGPEASAFTK KMVENAKKIE VEFNKGQRTD KYGRGLAYIY ADGKMVNEAL VRQGLAKVAY VYKPNNTHEQ HLRKSEAQAK KEKLNIWSEN DADSGQ

Sample

Temperature 310 K, pH 6.4



Release date
1995-07-30
Citation
Deletion of the omega-loop in the active site of staphylococcal nuclease. 2. Effects on protein structure and dynamics
Baldisseri, D.M., Torchia, D.A., Poole, L.B., Gerlt, J.A.
Biochemistry (1991), 30, 3628-3633, PubMed 2015220 , DOI 10.1021/bi00229a006 ,
Related entities 1. micrococcal nuclease, : 1 : 115 entities Detail