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BMRB: 15813

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1H, 13C and 15N resonance assignments of the ribosome binding domain of E. coli Trigger Factor

Authors

Hsu, S., Cabrita, L.D., Christodoulou, J., Dobson, C.M.

Assembly

E. coli Trigger Factor (TF) Ribosome Binding Domain (RBD)

Entity

1. E. coli Trigger Factor (TF) Ribosome Binding Domain (RBD) (polymer, Thiol state: not present), 117 monomers, 12938.69 × 2 Da Detail

MQVSVETTQG LGRRVTITIA ADSIETAVKS ELVNVAKKVR IDGFRKGKVP MNIVAQRYGA SVRQDVLGDL MSRNFIDAII KEKINPAGAP TYVPGEYKLG EDFTYSVEFE VYPEVEL

Total weight

25877.38 Da

Max. entity weight

12938.69 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.2 %, Completeness (bb): 97.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.2 % (1220 of 1353)	89.7 % (629 of 701)	89.3 % (475 of 532)	96.7 % (116 of 120)
Backbone	97.5 % (675 of 692)	95.4 % (227 of 238)	99.1 % (339 of 342)	97.3 % (109 of 112)
Sidechain	84.9 % (653 of 769)	86.8 % (402 of 463)	81.9 % (244 of 298)	87.5 % (7 of 8)
Aromatic	75.0 % (60 of 80)	75.0 % (30 of 40)	75.0 % (30 of 40)
Methyl	96.1 % (146 of 152)	96.1 % (73 of 76)	96.1 % (73 of 76)

1. E. coli TF-RBD

MQVSVETTQG LGRRVTITIA ADSIETAVKS ELVNVAKKVR IDGFRKGKVP MNIVAQRYGA SVRQDVLGDL MSRNFIDAII KEKINPAGAP TYVPGEYKLG EDFTYSVEFE VYPEVEL

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.35, Details TF-RBD 1-117 in 10mM Tris, 200mM (NH4)2.SO4, pH 7.35, 298K

#	Name	Isotope labeling	Concentration
1	E. coli TF-RBD	[U-98% 13C; U-98% 15N]	0.2 mM
2	Tris	natural abundance	10 uM
3	(NH4)2.SO4		200 mM
4	H2O	natural abundance	90 %
5	D2O	[U-100% 2H]	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.755 ppm	internal	direct	1.0
¹⁵N	water	protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.755 ppm	internal	direct	1.0
¹⁵N	water	protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.755 ppm	internal	direct	1.0
¹⁵N	water	protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.755 ppm	internal	direct	1.0
¹⁵N	water	protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.17 ppm, Outliers: 3 Detail

Release date

2008-11-24

Citation 1

1H, 15N and 13C assignments of the dimeric ribosome binding domain of trigger factor from Escherichia coli
Hsu, S.D., Dobson, C.M.
Biomol. NMR Assign. (2009), 3, 17-20, PubMed 19636937 , DOI 10.1007/s12104-008-9130-8 , Abstract

Show more 1 citaion

Citation 2

Chaperone binding at the ribosomal exit tunnel
Kristensen, O., Gajhede, M.
Structure (2003), 11, 1547-1556, PubMed 14656439 , DOI: , Abstract

Hide non-primary 1 citaion

Related entities 1. E. coli Trigger Factor (TF) Ribosome Binding Domain (RBD), : 1 : 18 : 6 : 100 entities Detail

Interaction partners 1. E. coli Trigger Factor (TF) Ribosome Binding Domain (RBD), : 44 interactors Detail

More details for 44 interactors identified by 5 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC