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BMRB: 15814

1WLH

1H, 13C and 15N resonance assignments of the domain 5 of ABP-120 from Dictyostelium in native and 8M urea-denatured states.

Authors

Hsu, S., Cabrita, L.D., Christodoulou, J., Dobson, C.M.

Assembly

Domain 5 of Dictyostelium ABP-120

Entity

1. Domain 5 of Dictyostelium ABP-120 (polymer, Thiol state: all free), 108 monomers, 11371.50 Da Detail

MTVKPAPSAE HSYAEGEGLV KVFDNAPAEF TIFAVDTKGV ARTDGGDPFE VAINGPDGLV VDAKVTDNND GTYGVVYDAP VEGNYNVNVT LRGNPIKNMP IDVKCIEG

Formula weight

11371.5 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 95.4 %, Completeness: 54.2 %, Completeness (bb): 91.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	54.2 % (631 of 1164)	40.3 % (240 of 595)	64.1 % (295 of 460)	88.1 % (96 of 109)
Backbone	91.3 % (577 of 632)	88.2 % (194 of 220)	92.3 % (288 of 312)	95.0 % (95 of 100)
Sidechain	22.1 % (139 of 628)	12.3 % (46 of 375)	37.7 % (92 of 244)	11.1 % (1 of 9)
Aromatic	5.3 % (4 of 76)	10.5 % (4 of 38)	0.0 % (0 of 38)
Methyl	18.3 % (23 of 126)	14.3 % (9 of 63)	22.2 % (14 of 63)

1. Domain 5 of Dictyostelium ABP-120

MTVKPAPSAE HSYAEGEGLV KVFDNAPAEF TIFAVDTKGV ARTDGGDPFE VAINGPDGLV VDAKVTDNND GTYGVVYDAP VEGNYNVNVT LRGNPIKNMP IDVKCIEG

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.6, Details Domain 5 of ABP in binding buffer (10mM Hepes (pH 7.6), 140mM NH4Cl, 6mM MgCl2, 0.005mM spermine, 2mM spermidine, 2mM DTT) and 8M urea

#	Name	Isotope labeling	Concentration
1	Domain 5 of Dictyostelium ABP-120	[U-98% 13C; U-98% 15N]	0.4 mM
2	D2O	natural abundance	5 %
3	H2O	natural abundance	95 %
4	DTT	natural abundance	2 mM
5	HEPES	natural abundance	10 mM
6	urea	natural abundance	8 M

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.6

#	Name	Isotope labeling	Concentration
7	Domain 5 of Dictyostelium ABP-120	[U-98% 13C; U-98% 15N]	0.4 mM
8	beta-mercaptoethanol	natural abundance	2 mM
9	potassium phosphate	natural abundance	20 mM
10	D2O	natural abundance	5 %
11	H2O	natural abundance	95 %

Chem. Shift Complete2

Sequence coverage: 99.1 %, Completeness: 71.2 %, Completeness (bb): 94.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	71.2 % (1658 of 2328)	63.4 % (755 of 1190)	77.0 % (708 of 920)	89.4 % (195 of 218)
Backbone	94.1 % (1189 of 1264)	92.7 % (408 of 440)	94.4 % (589 of 624)	96.0 % (192 of 200)
Sidechain	51.6 % (648 of 1256)	46.3 % (347 of 750)	61.1 % (298 of 488)	16.7 % (3 of 18)
Aromatic	50.0 % (76 of 152)	55.3 % (42 of 76)	44.7 % (34 of 76)
Methyl	57.5 % (145 of 252)	55.6 % (70 of 126)	59.5 % (75 of 126)

1. Domain 5 of Dictyostelium ABP-120

MTVKPAPSAE HSYAEGEGLV KVFDNAPAEF TIFAVDTKGV ARTDGGDPFE VAINGPDGLV VDAKVTDNND GTYGVVYDAP VEGNYNVNVT LRGNPIKNMP IDVKCIEG

Show sample condition

Sample #1

#	Name	Isotope labeling	Concentration
1	Domain 5 of Dictyostelium ABP-120	[U-98% 13C; U-98% 15N]	0.4 mM
2	D2O	natural abundance	5 %
3	H2O	natural abundance	95 %
4	DTT	natural abundance	2 mM
5	HEPES	natural abundance	10 mM
6	urea	natural abundance	8 M

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.6

#	Name	Isotope labeling	Concentration
7	Domain 5 of Dictyostelium ABP-120	[U-98% 13C; U-98% 15N]	0.4 mM
8	beta-mercaptoethanol	natural abundance	2 mM
9	potassium phosphate	natural abundance	20 mM
10	D2O	natural abundance	5 %
11	H2O	natural abundance	95 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.45 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.45 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 1.1 ppm, Outliers: 2 Detail

Release date

2009-01-05

Citation

1H, 15N and 13C assignments of domain 5 of Dictyostelium discoideum gelation factor (ABP-120) in its native and 8M urea-denatured states
Hsu, S., Cabrita, L.D., Christodoulou, J., Dobson, C.M.
Biomol. NMR Assign. (2009), 3, 29-31, PubMed 19636940 , DOI 10.1007/s12104-008-9134-4 , Abstract