BMRBj - BMREntryMaster

Found 1 Document (1.189 seconds)

BMRB: 1582

Deletion of the omega-loop in the Active Site of Staphylococcal Nuclease 2. Effects on Protein Structure and Dynamics

Authors

Baldisseri, D.M., Torchia, D.A., Poole, L.B., Gerlt, J.A.

Assembly

micrococcal nuclease

Source organism

Staphylococcus aureus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 83.3 %, Completeness: 26.9 %, Completeness (bb): 62.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	26.9 % (287 of 1066)	17.2 % (157 of 914)	85.5 % (130 of 152)
Backbone	62.1 % (267 of 430)	46.9 % (137 of 292)	94.2 % (130 of 138)
Sidechain	3.1 % (20 of 636)	3.2 % (20 of 622)	0.0 % (0 of 14)
Aromatic	0.0 % (0 of 58)	0.0 % (0 of 57)	0.0 % (0 of 1)
Methyl	8.5 % (6 of 71)	8.5 % (6 of 71)

1. micrococcal nuclease

XXXXXXXXXX XXKLHKEPAT LIKAIDGDTV KLMYKGQPMT FRLLLVDTPD TKHPKKGVEK YGPEASAFTK KMVENAKKIE VEFNKGQRTD KYGRGLAYIY ADGKMVNEAL VRQGLAKVAY VYKPNNTHEQ HLRKSEAQAK KEKLNIWSEN DADSGQ

Show sample condition

Sample

Temperature 310 K, pH 6.4

Release date

1995-07-30

Citation

Deletion of the omega-loop in the active site of staphylococcal nuclease. 2. Effects on protein structure and dynamics
Baldisseri, D.M., Torchia, D.A., Poole, L.B., Gerlt, J.A.
Biochemistry (1991), 30, 3628-3633, PubMed 2015220 , DOI: , Abstract

Related entities 1. micrococcal nuclease, : 127 entities Detail

Experiments performed 1 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr1582_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr1582_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chemical_shift_assignment_data_set_one	Warning	258		82.7 (chain: 1, length: 156)

Assigned chemical shifts

Saveframe: chemical_shift_assignment_data_set_one
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 156, Sequence coverage: 82.7%
- Total number of assignments
  - ¹H chemical shifts: 129
  - ¹⁵N chemical shifts: 129
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID
1	X
2	X
3	X
4	X
5	X
6	X
7	X
8	X
9	X
10	X
11	X
12	X

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Found 1 Document (1.189 seconds)

BMRB: 1582

Sample

Related entities 1. micrococcal nuclease, : 127 entities Detail

Experiments performed 1 experiments Detail

Instrument

Sample

Chemical shift validation 3 contents Detail

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	914	129	14.1
¹⁵N chemical shifts	157	129	82.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	292	129	44.2
¹⁵N chemical shifts	138	129	93.5