Search

CIN85 Sh3-C domain in complex with ubiquitin
Authors
Forman-Kay, J., Bezsonova, I.
Assembly
CIN85 Sh3-C domain/ubiquitin complex
Entity
1. CIN85 Sh3-C domain (polymer, Thiol state: not reported), 62 monomers, 7143.024 × 8 Da Detail

KSKDYCKVIF PYEAQNDDEL TIKEGDIVTL INKDCIDVGW WEGELNGRRG VFPDNFVKLL PP


2. ubiquitin (polymer, Thiol state: not reported), 76 monomers, 8610.822 × 8 Da Detail

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGC


Total weight
126030.766 Da
Max. entity weight
8610.822 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 45.7 %, Completeness: 14.6 %, Completeness (bb): 29.0 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All14.6 % (243 of 1666) 7.7 % (67 of 871)18.4 % (118 of 643)38.2 % (58 of 152)
Backbone29.0 % (233 of 804)21.2 % (57 of 269)29.2 % (118 of 404)44.3 % (58 of 131)
Sidechain 6.7 % (66 of 990) 1.7 % (10 of 602)15.3 % (56 of 367) 0.0 % (0 of 21)
Aromatic 0.0 % (0 of 102) 0.0 % (0 of 51) 0.0 % (0 of 49) 0.0 % (0 of 2)
Methyl 0.6 % (1 of 164) 0.0 % (0 of 82) 1.2 % (1 of 82)

1. CIN85 Sh3-C domain

KSKDYCKVIF PYEAQNDDEL TIKEGDIVTL INKDCIDVGW WEGELNGRRG VFPDNFVKLL PP

2. ubiquitin

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGC

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4


#NameIsotope labelingTypeConcentration
1entity_1natural abundance0.1-1.0 mM
2entity_2[U-100% 15N]0.1-5.0 mM
3sodium phosphatenatural abundance50 mM
4DTTnatural abundance2 mM
5DSSnatural abundance5 mM
6sodium chloridenatural abundance150 mM
7H2Onatural abundance90 %
8D2O[U-100% 2H]10 %
Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4


#NameIsotope labelingTypeConcentration
9entity_1[U-100% 15N]0.1-1.0 mM
10entity_2natural abundance0.1-5.0 mM
11sodium phosphatenatural abundance50 mM
12DTTnatural abundance2 mM
13DSSnatural abundance5 mM
14sodium chloridenatural abundance150 mM
15H2Onatural abundance90 %
16D2O[U-100% 2H]10 %
Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.4


#NameIsotope labelingTypeConcentration
17entity_1[U-100% 13C; U-100% 15N]1.0 mM
18sodium phosphatenatural abundance50 mM
19DTTnatural abundance2 mM
20DSSnatural abundance5 mM
21sodium chloridenatural abundance150 mM
22H2Onatural abundance90 %
23D2O[U-100% 2H]10 %

LACS Plot; CA
Referencing offset: 0.16 ppm, Outliers: 3 Detail
LACS Plot; CB
Referencing offset: 0.16 ppm, Outliers: 3 Detail
Protein Blocks Logo
Calculated from 8 models in PDB: 2K6D, Strand ID: A, B Detail


Release date
2008-10-07
Citation
Interactions between the three CIN85 SH3 domains and ubiquitin: implications for CIN85 ubiquitination
Bezsonova, I., Bruce, C., Wiesner, S., Lin, H., Rotin, D., Forman-Kay, J.
Biochemistry (2008), 47, 8937-8949, PubMed 18680311 , DOI 10.1021/bi800439t ,
Related entities 1. CIN85 Sh3-C domain, : 1 : 2 : 1 : 250 entities Detail
Related entities 2. ubiquitin, : 1 entities Detail
Interaction partners 1. CIN85 Sh3-C domain, : 50 interactors Detail
Experiments performed 5 experiments Detail
Keywords CIN85, SH3 domain, ubiquitin