BMRBj - BMREntryMaster

Found 1 Document (3.535 seconds)

BMRB: 15885

6SLO

Backbone assignment of the UHM domain of Puf60 in the free form

Authors

Corsini, L., Sattler, M.

Assembly

UHM domain of Puf60

Entity

1. UHM domain of Puf60 (polymer, Thiol state: all free), 103 monomers, 11585.78 Da Detail

GAMESTVMVL RNMVDPKDID DDLEGEVTEE CGKFGAVNRV IIYQEKQGEE EDAEIIVKIF VEFSIASETH KAIQALNGRW FAGRKVVAEV YDQERFDNSD LSA

Formula weight

11585.78 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 37.6 %, Completeness (bb): 66.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	37.6 % (443 of 1177)	21.7 % (132 of 609)	46.4 % (212 of 457)	89.2 % (99 of 111)
Backbone	66.9 % (412 of 616)	50.9 % (108 of 212)	67.9 % (205 of 302)	97.1 % (99 of 102)
Sidechain	19.2 % (126 of 657)	6.0 % (24 of 397)	40.6 % (102 of 251)	0.0 % (0 of 9)
Aromatic	0.0 % (0 of 82)	0.0 % (0 of 41)	0.0 % (0 of 40)	0.0 % (0 of 1)
Methyl	12.9 % (15 of 116)	8.6 % (5 of 58)	17.2 % (10 of 58)

1. UHM domain of Puf60

GAMESTVMVL RNMVDPKDID DDLEGEVTEE CGKFGAVNRV IIYQEKQGEE EDAEIIVKIF VEFSIASETH KAIQALNGRW FAGRKVVAEV YDQERFDNSD LSA

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 300 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	UHM domain of Puf60	[U-100% 13C; U-100% 15N]	0.4 (±0.1) mM
2	NaH2PO4	natural abundance	50 mM
3	NaCl	natural abundance	150 mM
4	b-mercaptoethanol	natural abundance	5 mM
5	H2O	natural abundance	90 %
6	D2O	[U-100% 2H]	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.3 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.3 ppm, Outliers: 1 Detail

Release date

2008-10-14

Citation

Backbone assignment of the UHM domain of Puf60 free and bound to five ligands
Corsini, L., Sattler, M.
Biomol. NMR Assign. (2008), 2, 211-214, PubMed 19636907 , DOI 10.1007/s12104-008-9123-7 , Abstract

Related entities 1. UHM domain of Puf60, : 1 : 8 : 134 entities Detail

Interaction partners 1. UHM domain of Puf60, : 99 interactors Detail

More details for 99 interactors identified by 24 citations

Experiments performed 2 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr15885_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr15885_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	395		99.0 (chain: 1, length: 103)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 103, Sequence coverage: 99.0%
- Total number of assignments
  - ¹³C chemical shifts: 197
  - ¹H chemical shifts: 99
  - ¹⁵N chemical shifts: 99
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	457	197	43.1
¹H chemical shifts	609	99	16.3
¹⁵N chemical shifts	116	99	85.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	206	102	49.5
¹H chemical shifts	212	99	46.7
¹⁵N chemical shifts	102	99	97.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	251	95	37.8
¹H chemical shifts	397	0	0.0
¹⁵N chemical shifts	14	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	61	8	13.1
¹H chemical shifts	61	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	40	0	0.0
¹H chemical shifts	41	0	0.0
¹⁵N chemical shifts	1	0	0.0

Keywords alternative splicing, FBP interacting repressor, RRM, splicing, UHM, UHM ligand motif, ULM

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Found 1 Document (3.535 seconds)

BMRB: 15885

Sample

Chemical shift reference

Chemical shift reference

Related entities 1. UHM domain of Puf60, : 1 : 8 : 134 entities Detail

Interaction partners 1. UHM domain of Puf60, : 99 interactors Detail

Experiments performed 2 experiments Detail

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail