BMRB: 15887

1H, 15N chemical shifts of the UHM domain of Puf60 in complex with U2AF65(85-112)

Authors

Corsini, L., Sattler, M.

Assembly

minimal complex of Puf60 and U2AF65

Entity

1. UHM domain of Puf60 (polymer, Thiol state: all free), 110 monomers, 12636.97 Da Detail

MKHHHHHHPM ESTVMVLRNM VDPKDIDDDL EGEVTEECGK FGAVNRVIIY QEKQGEEEDA EIIVKIFVEF SIASETHKAI QALNGRWFAG RKVVAEVYDQ ERFDNSDLSA

2. U2AF65(85-112) (polymer, Thiol state: not present), 36 monomers, 4341.931 Da Detail

KKKVRKYWDV PPPGFEHITP MQYKAMQAGS HHHHHH

Total weight

16978.9 Da

Max. entity weight

12636.97 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 58.9 %, Completeness: 18.7 %, Completeness (bb): 41.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	18.7 % (197 of 1056)	12.3 % (111 of 904)	56.6 % (86 of 152)
Backbone	41.0 % (178 of 434)	31.3 % (92 of 294)	61.4 % (86 of 140)
Sidechain	3.1 % (19 of 622)	3.1 % (19 of 610)	0.0 % (0 of 12)
Aromatic	0.0 % (0 of 88)	0.0 % (0 of 86)	0.0 % (0 of 2)
Methyl	1.5 % (1 of 66)	1.5 % (1 of 66)

1. UHM domain of Puf60

MKHHHHHHPM ESTVMVLRNM VDPKDIDDDL EGEVTEECGK FGAVNRVIIY QEKQGEEEDA EIIVKIFVEF SIASETHKAI QALNGRWFAG RKVVAEVYDQ ERFDNSDLSA

2. U2AF65(85-112)

KKKVRKYWDV PPPGFEHITP MQYKAMQAGS HHHHHH

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 295 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	UHM domain of Puf60	[U-100% 13C; U-100% 15N]		0.177 (±0.01) mM
2	U2AF65(85-112)	natural abundance		0.333 (±0.01) mM
3	NaH2PO4	natural abundance		50 mM
4	NaCl	natural abundance		150 mM
5	b-mercaptoethanol	natural abundance		5 mM
6	H2O	natural abundance		90 %
7	D2O	[U-100% 2H]		10 %

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Release date

2008-10-14

Citation

Backbone assignment of the UHM domain of Puf60 free and bound to five ligands
Corsini, L., Sattler, M.
Biomol. NMR Assign. (2008), 2, 211-214, PubMed 19636907 , DOI 10.1007/s12104-008-9123-7 , Abstract

Related entities 1. UHM domain of Puf60, : 1 : 74 entities Detail

Related entities 2. U2AF65(85-112), : 7 entities Detail

Experiments performed 1 experiments Detail

Chemical shift validation 4 contents Detail