BMRB: 15888

1H, 15N chemical shifts of the UHM domain of Puf60 in complex with SF1(1-25)

Authors

Corsini, L., Sattler, M.

Assembly

minimal complex of Puf60 and SF1

Entity

1. UHM domain of Puf60 (polymer, Thiol state: all free), 110 monomers, 12636.97 Da Detail

MKHHHHHHPM ESTVMVLRNM VDPKDIDDDL EGEVTEECGK FGAVNRVIIY QEKQGEEEDA EIIVKIFVEF SIASETHKAI QALNGRWFAG RKVVAEVYDQ ERFDNSDLSA

2. SF1(1-25) (polymer, Thiol state: not present), 25 monomers, 2876.212 Da Detail

MATGANATPL DFPSKKRKRS RWNQD

Total weight

15513.182 Da

Max. entity weight

12636.97 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 68.9 %, Completeness: 20.6 %, Completeness (bb): 47.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	20.6 % (199 of 967)	12.9 % (106 of 823)	64.6 % (93 of 144)
Backbone	47.3 % (191 of 404)	35.9 % (98 of 273)	71.0 % (93 of 131)
Sidechain	1.4 % (8 of 563)	1.5 % (8 of 550)	0.0 % (0 of 13)
Aromatic	3.0 % (2 of 66)	3.1 % (2 of 64)	0.0 % (0 of 2)
Methyl	7.8 % (5 of 64)	7.8 % (5 of 64)

1. UHM domain of Puf60

MKHHHHHHPM ESTVMVLRNM VDPKDIDDDL EGEVTEECGK FGAVNRVIIY QEKQGEEEDA EIIVKIFVEF SIASETHKAI QALNGRWFAG RKVVAEVYDQ ERFDNSDLSA

2. SF1(1-25)

MATGANATPL DFPSKKRKRS RWNQD

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Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 295 (±0.1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	UHM domain of Puf60	[U-100% 13C; U-100% 15N]		0.177 (±0.01) mM
2	SF1(1-25)	natural abundance		0.333 (±0.01) mM
3	NaH2PO4	natural abundance		50 mM
4	NaCl	natural abundance		150 mM
5	b-mercaptoethanol	natural abundance		5 mM
6	H2O	natural abundance		95 %
7	D2O	[U-100% 2H]		5 %

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Release date

2008-10-14

Citation

Backbone assignment of the UHM domain of Puf60 free and bound to five ligands
Corsini, L., Sattler, M.
Biomol. NMR Assign. (2008), 2, 211-214, PubMed 19636907 , DOI 10.1007/s12104-008-9123-7 , Abstract

Entries sharing articles BMRB: 5 entries Detail

Related entities 1. UHM domain of Puf60, : 1 : 74 entities Detail

Related entities 2. SF1(1-25), : 1 : 5 : 1 entities Detail

Interaction partners 2. SF1(1-25), : 155 interactors Detail

Experiments performed 1 experiments Detail

Chemical shift validation 4 contents Detail