1H, 15N chemical shifts of the UHM domain of Puf60 in complex with Prp16(201-238)
MKHHHHHHPM ESTVMVLRNM VDPKDIDDDL EGEVTEECGK FGAVNRVIIY QEKQGEEEDA EIIVKIFVEF SIASETHKAI QALNGRWFAG RKVVAEVYDQ ERFDNSDLSA
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 19.5 % (207 of 1060) | 12.2 % (110 of 903) | 61.8 % (97 of 157) |
Backbone | 43.8 % (198 of 452) | 32.9 % (101 of 307) | 66.9 % (97 of 145) |
Sidechain | 1.6 % (10 of 608) | 1.7 % (10 of 596) | 0.0 % (0 of 12) |
Aromatic | 6.8 % (5 of 74) | 7.0 % (5 of 71) | 0.0 % (0 of 3) |
Methyl | 0.0 % (0 of 63) | 0.0 % (0 of 63) |
1. UHM domain of Puf60
MKHHHHHHPM ESTVMVLRNM VDPKDIDDDL EGEVTEECGK FGAVNRVIIY QEKQGEEEDA EIIVKIFVEF SIASETHKAI QALNGRWFAG RKVVAEVYDQ ERFDNSDLSA2. Prp16(201-238)
GAMGRHRPKD AATPSRSTWE EEDSGYGSSR RSQWESPSPT PSSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 295 (±0.1) K, pH 6.8 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | UHM domain of Puf60 | [U-100% 13C; U-100% 15N] | 0.129 (±0.01) mM | |
2 | Prp16(201-238) | natural abundance | 1.93 (±0.01) mM | |
3 | NaH2PO4 | natural abundance | 50 mM | |
4 | NaCl | natural abundance | 150 mM | |
5 | b-mercaptoethanol | natural abundance | 5 mM | |
6 | H2O | natural abundance | 95 % | |
7 | D2O | [U-100% 2H] | 5 % |