BMRB: 15907

1J coupling constants related to the Ca carbons in human Ubiquitin

Authors

Schmidt, J.M., Lohr, F.

Assembly

Ubiquitin

Entity

1. Ubiquitin (polymer, Thiol state: not present), 76 monomers, 8564.731 Da Detail

MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG

Formula weight

8564.731 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

coupling_constants

Coupling constant

265 J values in 4 lists
Field strength (¹H) 600 MHz, 800 MHz, Pressure 1 atm, Temperature 300 K, pH 7.6 Detail

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Pressure 1 atm, Temperature 300 K, pH 7.6

Experiment name 2D HN(COCA-J), 3D Ca-coupled [15N,1H]-TROSY-HNCO, 2D IPAP-type HN(CO-a/b-NCa-J)-TROSY, 3D Cb-coupled [15N,1H]-TROSY-HN(CO)CA, 3D 1Ha-13Ca multiple-quantum HCAN quantitative J correlation, 3D Ha-coupled ct-[15N,1H]-TROSY-HN(CO)CA

List #1 1JCaCO, Field strength (¹H) 800 MHz

List #2 1JCaN_, Field strength (¹H) 600 MHz

List #3 1JCaHa, Field strength (¹H) 800 MHz, Details 28-ms constant-time Ca evolution in 3D TROSY experiments 2D on FRXN only

List #4 1JCaCb, Field strength (¹H) 800 MHz, Details Ca evolution time typically 70-90ms

Release date

2008-11-18

Citation 1

Variation in protein C(alpha)-related one-bond J couplings
Schmidt, J.M., Howard, M.J., Maestre-Martinez, M., Perez, C.S., Lohr, F.
Magn. Reson. Chem. (2009), 47, 16-30, PubMed 18853398 , DOI 10.1002/mrc.2337 , Abstract

Show more 8 citaions

Citation 2

Sequential backbone assignment of isotopically enriched proteins in D2O by deuterium-decoupled HA(CA)N and HA(CACO)N
Wang, A.C., Grzesiek, S., Tschudin, R., Lodi, P.J., Bax, A.
J. Biomol. NMR (1995), 5, 376-382, PubMed 7647557 , DOI: , Abstract

Citation 3

TROSY in triple-resonance experiments: new perspectives for sequential NMR assignment of large proteins
Salzmann, M., Pervushin, K., Wider, G., Senn, H., Wuthrich, K.
Proc. Natl. Acad. Sci. U. S. A. (1998), 95, 13585-13590, PubMed 9811843 , Abstract

Citation 4

Recording heteronuclear quantitative J-correlation spectra with internal reference peaks
Lohr, F., Perez, C., Schmidt, J.M., Ruterjans, H.
Bull. Magn. Reson. (1999), 20, 9-14

Citation 5

HN(alpha/beta-COCA-J) experiment for measurement of (1)J(C'C(alpha)) couplings from two-dimensional
Permi, P., Sorsa, T., Kilpelainen, I., Annila, A.
J. Magn. Reson. (1999), 141, 44-51, PubMed 10527742 , DOI 10.1006/jmre.1999.1886 , Abstract

Citation 6

TROSY-type triple-resonance experiments for sequential NMR assignments of large proteins
Salzmann, M., Wider, G., Pervushin, K., Senn, H., Wuthrich, K.
J. Am. Chem. Soc. (1999), 121, 844-848, DOI 10.1021/ja9834226

Citation 7

TROSY-based HNCO pulse sequence for the measurement of 1HN-15N, 15N-13CO, 1HN-13CO, 13CO-13Ca and 1HN-13Ca dipolar couplings in 15N, 13C, 2H-labeled proteins
Yang, D., Venters, R.A., Mueller, G.A., Choy, W.Y., Kay, L.E.
J. Biomol. NMR (1999), 14, 333-343

Citation 8

Transverse relaxation optimised spin-state selective NMR experiments for measurement of residual dipolar couplings
Permi, P., Annila, A.
J. Biomol. NMR (2000), 16, 221-227, PubMed 10805128 , Abstract

Citation 9

Measurement of (13)C(alpha)-(13)C(beta) dipolar couplings in (15)N,(13)C,(2)H-labeled proteins: application to domain orientation in maltose binding protein
Evenas, J., Mittermaier, A., Yang, D., Kay, L.E.
J. Am. Chem. Soc. (2001), 123, 2858-2864, PubMed 11456973 , DOI: , Abstract

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Entries sharing articles BMRB: 13 entries Detail

Related entities 1. Ubiquitin, : 1 : 292 : 11 : 71 : 321 entities Detail

Interaction partners 1. Ubiquitin, : 27 interactors Detail

Experiments performed 6 experiments Detail