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BMRB: 15930

6N61

Sequence-specific 1H, 15N, and 13C resonance assignments and relaxation parameters for the whole region 4 of Escherichia coli RNA polymerase sigma70 subunit in 10% TFE

Authors

Kaczka, P., Polkowska, A.

Assembly

4 sigma70

Entity

1. 4 sigma70 (polymer, Thiol state: not present), 107 monomers, 12163.63 Da Detail

MGSSHHHHHH SSGLVPRGSH MLELPLDSAT TESLRAATHD VLAGLTAREA KVLRMRFGID MNTDYTLEEV GKQFDVTRER IRQIEAKALR KLRHPSRSEV LRSFLDD

Formula weight

12163.63 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 59.2 %, Completeness (bb): 78.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	59.2 % (724 of 1222)	52.2 % (332 of 636)	60.8 % (291 of 479)	94.4 % (101 of 107)
Backbone	78.3 % (498 of 636)	92.6 % (201 of 217)	62.2 % (196 of 315)	97.1 % (101 of 104)
Sidechain	46.4 % (319 of 687)	31.3 % (131 of 419)	70.9 % (188 of 265)	0.0 % (0 of 3)
Aromatic	0.0 % (0 of 74)	0.0 % (0 of 37)	0.0 % (0 of 37)
Methyl	52.5 % (62 of 118)	27.1 % (16 of 59)	78.0 % (46 of 59)

1. 4 sigma70

MGSSHHHHHH SSGLVPRGSH MLELPLDSAT TESLRAATHD VLAGLTAREA KVLRMRFGID MNTDYTLEEV GKQFDVTRER IRQIEAKALR KLRHPSRSEV LRSFLDD

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 4.55

#	Name	Isotope labeling	Type	Concentration
1	4 sigma70	[U-13C; U-15N]		3 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.21 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.21 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

Heteronucl. T₁

101 T₁ values in 1 lists
Coherence Sz, Field strength (¹H) 500 MHz, Pressure 1 atm, Temperature 298 K, pH 4.55 Detail

Heteronucl. T₂

101 T₂ values in 1 lists
Coherence S(+,-), Field strength (¹H) 500 MHz, Pressure 1 atm, Temperature 298 K, pH 4.55 Detail

Heteronucl. T₁/T₂

101 T₁/T₂ values in 1 lists
Field strength (¹H) 500 MHz, Pressure 1 atm, Temperature 298 K, pH 4.55 Detail

Release date

2012-01-03

Citation

Backbone dynamics of TFE-induced native-like fold of region 4 of Escherichia coli RNA polymerase sigma70 subunit
Kaczka, P., Polkowska, A., Bolewska, K., Zhukov, I., Poznanski, J., Wierzchowski, K.L.
Proteins (2010), 78, 754-768, PubMed 19847776 , DOI 10.1002/prot.22607 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 15975 released on 2009-12-13
    Title Sequence-specific 1H, 15N, and 13C resonance assignments and relaxation parameters for the whole region 4 of Escherichia coli RNA polymerase sigma70 subunit in 30% TFE
  BMRB: 15936 released on 2009-11-19
    Title Sequence-specific 1H, 15N, resonance assignments of nine 1H-{15N} HSQC spectras for the whole region 4 of Escherichia coli RNA polymerase sigma70 subunit recorded at TFE concentrations increasing in the range of 0-30%

Related entities 1. 4 sigma70, : 1 : 94 : 18 entities Detail

Interaction partners 1. 4 sigma70, : 32 interactors Detail

More details for 32 interactors identified by 5 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC