BMRBj - BMREntryMaster

Found 1 Document (0.957 seconds)

BMRB: 15975

6N61

Sequence-specific 1H, 15N, and 13C resonance assignments and relaxation parameters for the whole region 4 of Escherichia coli RNA polymerase sigma70 subunit in 30% TFE

Authors

Kaczka, P., Poznanski, J.

Assembly

4 sigma70

Entity

1. 4 sigma70 (polymer, Thiol state: not present), 107 monomers, 12163.63 Da Detail

MGSSHHHHHH SSGLVPRGSH MLELPLDSAT TESLRAATHD VLAGLTAREA KVLRMRFGID MNTDYTLEEV GKQFDVTRER IRQIEAKALR KLRHPSRSEV LRSFLDD

Formula weight

12163.63 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, heteronucl_NOEs, heteronucl_T1_relaxation, heteronucl_T2_relaxation

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 88.2 %, Completeness (bb): 95.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.2 % (1078 of 1222)	85.1 % (541 of 636)	90.4 % (433 of 479)	97.2 % (104 of 107)
Backbone	95.6 % (608 of 636)	91.7 % (199 of 217)	97.8 % (308 of 315)	97.1 % (101 of 104)
Sidechain	83.0 % (570 of 687)	81.6 % (342 of 419)	84.9 % (225 of 265)	100.0 % (3 of 3)
Aromatic	0.0 % (0 of 74)	0.0 % (0 of 37)	0.0 % (0 of 37)
Methyl	96.6 % (114 of 118)	93.2 % (55 of 59)	100.0 % (59 of 59)

1. 4 sigma70

MGSSHHHHHH SSGLVPRGSH MLELPLDSAT TESLRAATHD VLAGLTAREA KVLRMRFGID MNTDYTLEEV GKQFDVTRER IRQIEAKALR KLRHPSRSEV LRSFLDD

Show sample condition

Sample

Solvent system trifluoroethanol/water, Pressure 1 atm, Temperature 298 K, pH 4.55, Details 30% TFE (2,2,2-trifluoroehtanol)

#	Name	Isotope labeling	Type	Concentration
1	4 sigma70	[U-13C; U-15N]		3 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.14 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.14 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 2 Detail

Heteronucl. T₁

101 T₁ values in 1 lists
Coherence Sz, Field strength (¹H) 800 MHz, Pressure 1 atm, Temperature 298 K, pH 4.55 Detail

Heteronucl. T₂

101 T₂ values in 1 lists
Coherence S(+,-), Field strength (¹H) 800 MHz, Pressure 1 atm, Temperature 298 K, pH 4.55 Detail

Heteronucl. NOE

100 NOE values in 1 lists
Value type peak height, Field strength (¹H) 800 MHz, Pressure 1 atm, Temperature 298 K, pH 4.55 Detail

Heteronucl. T₁/T₂

101 T₁/T₂ values in 1 lists
Field strength (¹H) 800 MHz, Pressure 1 atm, Temperature 298 K, pH 4.55 Detail

Release date

2009-12-13

Citation

Backbone dynamics of TFE-induced native-like fold of region 4 of Escherichia coli RNA polymerase sigma70 subunit
Kaczka, P., Polkowska-Nowakowska, A., Bolewska, K., Zhukov, I., Poznaski, J., Wierzchowski, K.L.
Proteins (2010), 78, 754-768, PubMed 19847776 , DOI 10.1002/prot.22607 , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 15930 released on 2012-01-03
    Title Sequence-specific 1H, 15N, and 13C resonance assignments and relaxation parameters for the whole region 4 of Escherichia coli RNA polymerase sigma70 subunit in 10% TFE
  BMRB: 15936 released on 2009-11-19
    Title Sequence-specific 1H, 15N, resonance assignments of nine 1H-{15N} HSQC spectras for the whole region 4 of Escherichia coli RNA polymerase sigma70 subunit recorded at TFE concentrations increasing in the range of 0-30%

Related entities 1. 4 sigma70, : 1 : 94 : 18 entities Detail

Interaction partners 1. 4 sigma70, : 32 interactors Detail

More details for 32 interactors identified by 5 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC