Search

NMR Spectroscopic Characterization of 13C, 15N-labeled Ribonuclease A in urea 8M
Authors
Lopez-Alonso, J.P., Bruix, M., Font, J., Ribo, M., Vilanova, M., Morbio, M., Jimenez, M.A., Rico, M., Santoro, J., Gonzalez, C., Laurents, D.V.
Assembly
bovine pancreas ribonuclease A
Entity
1. bovine pancreas ribonuclease A (polymer, Thiol state: all disulfide bound), 124 monomers, 13690.17 Da Detail

KETAAAKFER QHMDSSTSAA SSSNYCNQMM KSRNLTKDRC KPVNTFVHES LADVQAVCSQ KNVACKNGQT NCYQSYSTMS ITDCRETGSS KYPNCAYKTT QANKHIIVAC EGNPYVPVHF DASV


Formula weight
13690.17 Da
Entity Connection
disulfide 4 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1disulfidesing1:CYS26:SG1:CYS84:SG
2disulfidesing1:CYS40:SG1:CYS95:SG
3disulfidesing1:CYS58:SG1:CYS110:SG
4disulfidesing1:CYS65:SG1:CYS72:SG

Source organism
Bos taurus
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 99.2 %, Completeness: 35.0 %, Completeness (bb): 63.6 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All35.0 % (483 of 1380)17.4 % (124 of 714)46.7 % (245 of 525)80.9 % (114 of 141)
Backbone63.6 % (466 of 733)45.5 % (111 of 244)65.6 % (242 of 369)94.2 % (113 of 120)
Sidechain17.3 % (133 of 768) 2.8 % (13 of 470)43.0 % (119 of 277) 4.8 % (1 of 21)
Aromatic 0.0 % (0 of 94) 0.0 % (0 of 47) 0.0 % (0 of 47)
Methyl12.0 % (12 of 100) 0.0 % (0 of 50)24.0 % (12 of 50)

1. ribonuclease A

KETAAAKFER QHMDSSTSAA SSSNYCNQMM KSRNLTKDRC KPVNTFVHES LADVQAVCSQ KNVACKNGQT NCYQSYSTMS ITDCRETGSS KYPNCAYKTT QANKHIIVAC EGNPYVPVHF DASV

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 2.0 (±0.1)


#NameIsotope labelingTypeConcentration
1ribonuclease A[U-98% 13C; U-98% 15N]1.75 mM
2H2Onatural abundance90 %
3D2Onatural abundance10 %
4ureanatural abundance8 M

LACS Plot; CA
Referencing offset: -0.19 ppm, Outliers: 3 Detail
LACS Plot; CB
Referencing offset: -0.19 ppm, Outliers: 3 Detail
Release date
2010-10-17
Citation
NMR spectroscopy reveals that RNase A is chiefly denatured in 40% acetic acid: implications for oligomer formation by 3D domain swapping
Lopez-Alonso, J., Bruix, M., Font, J., Ribo, M., Vilanova, M., Jimenez, M., Santoro, J., Gonzalez, C., Laurents, D.V.
J. Am. Chem. Soc. (2010), 132, 1621-1630, PubMed 20085318 , DOI 10.1021/ja9081638 ,
Related entities 1. bovine pancreas ribonuclease A, : 1 : 101 : 94 entities Detail
Interaction partners 1. bovine pancreas ribonuclease A, : 8 interactors Detail