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ENHANCING THE ACTIVITY OF INSULIN BY STEREOSPECIFIC UNFOLDING
Authors
Hua, Q.X., Xu, B., Huang, K., Hu, S.Q., Nakarawa, S., Jia, W.H., Philips, N.F.PHIL., Wittaker, L., Wittaker, J., Katsoyannis, P.G., Weiss, M.A.
Assembly
INSULIN A CHAIN, INSULIN B CHAIN
Entity
1. INSULIN A CHAIN (polymer, Thiol state: all disulfide bound), 21 monomers, 2383.696 Da Detail

GIVEQCCTSI CSLYQLENYC N


2. INSULIN B CHAIN (polymer, Thiol state: all disulfide bound), 30 monomers, 3331.772 Da Detail

FVNQHLCGSD LVEALYLVCG ERGAFYTKPT


Total weight
5715.468 Da
Max. entity weight
3331.772 Da
Source organism
Homo sapiens
Exptl. method
SOLUTION NMR
Refine. method
DISTANCE GEOMETRY, SIMULATED ANNEALING
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 98.0 %, Completeness: 89.5 %, Completeness (bb): 78.2 % Detail

Polymer type: polypeptide(L)

Total1H
All89.5 % (263 of 294)89.5 % (263 of 294)
Backbone78.2 % (79 of 101)78.2 % (79 of 101)
Sidechain95.3 % (184 of 193)95.3 % (184 of 193)
Aromatic89.3 % (25 of 28)89.3 % (25 of 28)
Methyl100.0 % (29 of 29)100.0 % (29 of 29)

1. INSULIN A CHAIN

GIVEQCCTSI CSLYQLENYC N

2. INSULIN B CHAIN

FVNQHLCGSD LVEALYLVCG ERGAFYTKPT

Sample

Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 7.4, Details 0.5-0.8 mM PROTEIN, 90% H2O/ 10% D2O AND 20% ACETIC ACID


#NameIsotope labelingTypeConcentration
1INSULIN A CHAINnatural abundance0.5-0.8 mM
2INSULIN B CHAINnatural abundance0.5-0.8 mM
3H2Onatural abundance90 %
4D2Onatural abundance10 %
5ACETIC ACIDnatural abundance20 %

Protein Blocks Logo
Calculated from 20 models in PDB: 2K91, Strand ID: A, B Detail


Release date
2009-04-16
Citation
Enhancing the activity of a protein by stereospecific unfolding: conformational life cycle of insulin and its evolutionary origins
Hua, Q.X., Xu, B., Huang, K., Hu, S.Q., Nakarawa, S., Jia, W., Wang, S.H., Wittaker, J., Katsoyannis, P.G., Weiss, M.A.
J. Biol. Chem. (2009), 284, 14586-14596, PubMed 19321436 , DOI 10.1074/jbc.M900085200 ,
Related entities 1. INSULIN A CHAIN, : 111 : 9 : 7 : 74 entities Detail
Related entities 2. INSULIN B CHAIN, : 1 : 115 : 83 entities Detail
Interaction partners 1. INSULIN A CHAIN, : 1 : 1 interactors Detail
Interaction partners 2. INSULIN B CHAIN, : 6 : 1 interactors Detail
Experiments performed 3 experiments Detail
Keywords HORMONE, INSULIN, MUTANT